DNA Supercoiling and the Lrp Protein Determine the Directionality of fim Switch DNA Inversion in Escherichia coli K-12

A Kelly, C Conway, T Ó Cróinín, SGJ Smith… - Journal of …, 2006 - Am Soc Microbiol
Site-specific recombinases of the integrase family usually require cofactors to impart
directionality in the recombination reactions that they catalyze. The FimB integrase inverts …

Interaction of the FimB Integrase with thefimS Invertible DNA Element in Escherichia coliIn Vivo and In Vitro

LS Burns, SGJ Smith, CJ Dorman - Journal of Bacteriology, 2000 - Am Soc Microbiol
The FimB protein is a site-specific recombinase that inverts the fimS genetic switch in
Escherichia coli. Based on amino acid sequence analysis alone, FimB has been assigned to …

Functional analysis of the FimE integrase of Escherichia coli K‐12: isolation of mutant derivatives with altered DNA inversion preferences

SGJ Smith, CJ Dorman - Molecular microbiology, 1999 - Wiley Online Library
Phase variable expression of type 1 fimbriae in Escherichia coli arises from a site‐specific
recombination event that inverts a short segment of chromosomal DNA carrying the …

DNA relaxation‐dependent phase biasing of the fim genetic switch in Escherichia coli depends on the interplay of H‐NS, IHF and LRP

CP Corcoran, CJ Dorman - Molecular microbiology, 2009 - Wiley Online Library
Reversible inversion of the DNA element fimS is responsible for the phase variable
expression of type 1 fimbriae in Escherichia coli. The FimB tyrosine integrase site‐specific …

Comparative analysis of FimB and FimE recombinase activity

N Holden, IC Blomfield, BE Uhlin, M Totsika… - …, 2007 - microbiologyresearch.org
FimB and FimE are site-specific recombinases, part of the λ integrase family, and invert a
314 bp DNA switch that controls the expression of type 1 fimbriae in Escherichia coli. FimB …

DNA sequence heterogeneity in Fim tyrosine‐integrase recombinase‐binding elements and functional motif asymmetries determine the directionality of the fim genetic switch in …

MP McCusker, EC Turner… - Molecular microbiology, 2008 - Wiley Online Library
Phase‐variable expression of type 1 fimbriae in Escherichia coli K‐12 involves inversion by
site‐specific recombination of a 314 bp sequence containing the promoter for fim structural …

DNA inversions in phages and bacteria

P Van de Putte, N Goosen - Trends in Genetics, 1992 - cell.com
The inversion enzymes of the FimI system of E. coli also belong to the integrase family.
Since the biological function of this genetic switch resembles that promoted by the members …

Orientational control of fimE expression in Escherichia coli

BK Sohanpal, HD Kulasekara, A Bonnen… - Molecular …, 2001 - Wiley Online Library
Phase‐variable expression of type 1 fimbriae is, in part, controlled by site‐specific DNA
inversion of the fim switch in Escherichia coli. Of the two fim recombinases (FimB and FimE) …

A Rho‐dependent phase‐variable transcription terminator controls expression of the FimE recombinase in Escherichia coli

SA Joyce, CJ Dorman - Molecular microbiology, 2002 - Wiley Online Library
The fim switch is a 314 bp segment of invertible chromosomal DNA that is responsible for
phase‐variable expression of type 1 fimbriae in Escherichia coli. The switch harbours the …

Modulation of the Sensitivity of FimB Recombination to Branched-Chain Amino Acids and Alanine in Escherichia coli K-12

M Lahooti, PL Roesch, IC Blomfield - Journal of bacteriology, 2005 - Am Soc Microbiol
Phase variation of type 1 fimbriae of Escherichia coli requires the site-specific recombination
of a short invertible element. Inversion is catalyzed by FimB (switching in either direction) or …