Over the past few years, establishment and adaptation of cell-based assays for drug
development and testing has become an important topic in high-throughput screening …

Although used in academic research for several decades, 3D culture models have long
been regarded expensive, cumbersome and unnecessary in drug development processes …

Introduction: For the past 30 years 2D-cell-based assay models have dominated preclinical
cancer drug discovery efforts. 2D-cell-based models fail to predict in vivo efficacy …

Evaluation of drug cytotoxicity traditionally relies on use of cell monolayers, which are easily
miniaturized to the 1536-well plate format. Three-dimensional (3D) cell culture models have …

Purpose: To give a state-of-the-art overview on the promise of three-dimensional (3-D)
culture systems for anticancer drug development, with particular emphasis on multicellular …

In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some
features of solid tumors, such as their spatial architecture, physiological responses …

Drug development and testing are a tedious and expensive process with a high degree of
uncertainty in the clinical success and preclinical validation of manufactured therapeutic …

The increasing interest in studying cells using more in vivo-like three-dimensional (3D)
microenvironments has created a need for advanced 3D screening platforms with enhanced …

Culture of cells as three-dimensional (3D) aggregates can enhance in vitro tests for basic
biological research as well as for therapeutics development. Such 3D culture models …

Cells, grown as monolayers (2D models), are routinely used as initial model systems for
evaluating the effectiveness and safety of libraries of molecules with potential as therapeutic …