Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform
C Gaillochet, A Peña Fernández, V Goossens… - Genome biology, 2023 - Springer
Background Testing an ever-increasing number of CRISPR components is challenging
when developing new genome engineering tools. Plant biotechnology has few high …
when developing new genome engineering tools. Plant biotechnology has few high …
CRISPR-Cas9 editing in maize: systematic evaluation of off-target activity and its relevance in crop improvement
J Young, G Zastrow-Hayes, S Deschamps… - Scientific reports, 2019 - nature.com
CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture
productivity, but the possibility of unintended off-target edits has evoked some concerns …
productivity, but the possibility of unintended off-target edits has evoked some concerns …
CRISPR–Cas12a base editors confer efficient multiplexed genome editing in rice
Many Cas9-derived base editors have been developed for precise C–to–T and A–to–G base
editing in plants (Molla et al., 2021). They are typically based on a SpCas9 nickase or its …
editing in plants (Molla et al., 2021). They are typically based on a SpCas9 nickase or its …
Application of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
AA Malzahn, X Tang, K Lee, Q Ren, S Sretenovic… - BMC biology, 2019 - Springer
Background CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …
features that have expanded genome editing capabilities. Cas12a-mediated genome editing …
CRISPR/Cas9-based gene editing using egg cell-specific promoters in Arabidopsis and soybean
CRISPR/Cas9-based systems are efficient genome editing tools in a variety of plant species
including soybean. Most of the gene edits in soybean plants are somatic and non …
including soybean. Most of the gene edits in soybean plants are somatic and non …
Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases
The development of CRISPR‐based editors recognizing distinct protospacer‐adjacent
motifs (PAMs), or having different spacer length/structure requirements broadens the range …
motifs (PAMs), or having different spacer length/structure requirements broadens the range …
Hypercompact CRISPR–Cas12j2 (CasΦ) enables genome editing, gene activation, and epigenome editing in plants
CRISPR-Cas9,-Cas12a,-Cas12b, and-Cas13 have been harnessed for genome
engineering in human and plant cells (Liu et al., 2022). However, the large size of these Cas …
engineering in human and plant cells (Liu et al., 2022). However, the large size of these Cas …
Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems
Y Zhang, Q Ren, X Tang, S Liu, AA Malzahn… - Nature …, 2021 - nature.com
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
regions. Comprehensive genome engineering requires simultaneous targeting of multiple …
Highly efficient genome editing in plant protoplasts by ribonucleoprotein delivery of CRISPR-Cas12a nucleases
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome
editing is a powerful approach for crop improvement. Traditional transformation methods …
editing is a powerful approach for crop improvement. Traditional transformation methods …
Recent advances in genome editing using CRISPR/Cas9
Y Ding, H Li, LL Chen, K Xie - Frontiers in plant science, 2016 - frontiersin.org
The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …
associated nuclease 9) system is a versatile tool for genome engineering that uses a guide …