A variety of sequence-specific nucleases (SSNs) have been successfully used for plant
genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR) …

The ability to modify complex genomes precisely to create specific mutants is the holy grail
of basic research and applied genetics in the postgenomic era. Programmable sequence …

The past decade has witnessed unprecedented development in genome engineering, a
process that enables targeted modification of genomes. The identification of sequence …

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

The increasing burden of the world population on agriculture requires the development of
more robust crops. Dissecting the basic biology that underlies plant development and stress …

The development of clustered regularly interspaced palindromic repeats (CRISPR)‐
associated protein (Cas) variants with a broader recognition scope is critical for further …

CRISPR/Cas9-mediated genome editing is an important and versatile technology in modern
biological research. Recent advancements include base-editing CRISPR tools that enable …

The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …