A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)
S Gasparis, M Kała, M Przyborowski, LA Łyżnik… - Plant methods, 2018 - Springer
Background Genome editing of monocot plants can be accomplished by using the
components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic …
components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic …
Achieving plant CRISPR targeting that limits off‐target effects
The CRISPR‐Cas9 system (clustered regularly interspaced short palindromic repeats with
associated Cas9 protein) has been used to generate targeted changes for direct …
associated Cas9 protein) has been used to generate targeted changes for direct …
Expanding the range of CRISPR/Cas9 genome editing in rice
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
The power of CRISPR‐Cas9‐induced genome editing to speed up plant breeding
HX Cao, W Wang, HTT Le… - International journal of …, 2016 - Wiley Online Library
Genome editing with engineered nucleases enabling site‐directed sequence modifications
bears a great potential for advanced plant breeding and crop protection. Remarkably, the …
bears a great potential for advanced plant breeding and crop protection. Remarkably, the …
An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana
Background Our knowledge of natural genetic variation is increasing at an extremely rapid
pace, affording an opportunity to come to a much richer understanding of how effects of …
pace, affording an opportunity to come to a much richer understanding of how effects of …
Efficient generation of stable, heritable gene edits in wheat using CRISPR/Cas9
RM Howells, M Craze, S Bowden, EJ Wallington - BMC plant biology, 2018 - Springer
Background The use of CRISPR/Cas9 systems could prove to be a valuable tool in crop
research, providing the ability to fully knockout gene function in complex genomes or to …
research, providing the ability to fully knockout gene function in complex genomes or to …
Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants
Background Cas12a (formerly known as Cpf1), the class II type V CRISPR nuclease, has
been widely used for genome editing in mammalian cells and plants due to its distinct …
been widely used for genome editing in mammalian cells and plants due to its distinct …
CRISPR for crop improvement: an update review
The availability of genome sequences for several crops and advances in genome editing
approaches has opened up possibilities to breed for almost any given desirable trait …
approaches has opened up possibilities to breed for almost any given desirable trait …
Knocking out consumer concerns and regulator's rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals
Knocking out consumer concerns and regulator’s rules: efficient use of CRISPR/Cas
ribonucleoprotein complexes for genome editing in cereals | Genome Biology Skip to main …
ribonucleoprotein complexes for genome editing in cereals | Genome Biology Skip to main …