[HTML][HTML] CRISPR/Cas9: a powerful tool for crop genome editing

G Song, M Jia, K Chen, X Kong, B Khattak, C Xie, A Li… - The crop journal, 2016 - Elsevier
The CRISPR/Cas9 technology is evolved from a type II bacterial immune system and
represents a new generation of targeted genome editing technology that can be applied to …

CRISPR/Cas-mediated base editing: technical considerations and practical applications

KA Molla, Y Yang - Trends in biotechnology, 2019 - cell.com
Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new
CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or …

A modular cloning toolkit for genome editing in plants

F Hahn, A Korolev, L Sanjurjo Loures, V Nekrasov - BMC plant biology, 2020 - Springer
Background CRISPR/Cas has recently become a widely used genome editing tool in
various organisms, including plants. Applying CRISPR/Cas often requires delivering …

CRISPR-Cas9 based plant genome editing: Significance, opportunities and recent advances

N Soda, L Verma, J Giri - Plant Physiology and Biochemistry, 2018 - Elsevier
Precise genome editing is a quantum leap in the field of plant sciences. Clustered regularly
interspaced short palindromic repeats (CRISPR) and its associated Cas9 protein have …

CRISPR-GE: a convenient software toolkit for CRISPR-based genome editing

X Xie, X Ma, Q Zhu, D Zeng, G Li, YG Liu - Molecular plant, 2017 - cell.com
Use of the clustered regularly interspaced short palindromic repeat (CRISPR)-associated
protein9 (Cas9) and Cpf1 systems in plants (Ma et al., 2016; Wang et al., 2017) involves …

Optimizing multiplex CRISPR/Cas9-based genome editing for wheat

W Wang, A Akhunova, S Chao, E Akhunov - BioRxiv, 2016 - biorxiv.org
Background CRISPR/Cas9-based genome editing holds great promise to accelerate the
development of improved crop varieties by providing a powerful tool to modify the genomic …

[HTML][HTML] Target base editing in soybean using a modified CRISPR/Cas9 system

Y Cai, L Chen, Y Zhang, S Yuan, Q Su… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
In recent years, the CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9
(CRISPR-associated 9) system has revolutionized functional genomic research and crop …

Expanding the plant genome editing toolbox with recently developed CRISPR–Cas systems

N Wada, K Osakabe, Y Osakabe - Plant Physiology, 2022 - academic.oup.com
Since its first appearance, CRISPR–Cas9 has been developed extensively as a
programmable genome-editing tool, opening a new era in plant genome engineering …

[HTML][HTML] Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

S Svitashev, C Schwartz, B Lenderts, JK Young… - Nature …, 2016 - nature.com
Targeted DNA double-strand breaks have been shown to significantly increase the
frequency and precision of genome editing. In the past two decades, several double-strand …

Designed nucleases for targeted genome editing

J Lee, JH Chung, HM Kim, DW Kim… - Plant biotechnology …, 2016 - Wiley Online Library
Targeted genome‐editing technology using designed nucleases has been evolving rapidly,
and its applications are widely expanding in research, medicine and biotechnology. Using …