CRISPR-Cas in agriculture: Opportunities and challenges
S Kumar, LA Rymarquis, H Ezura… - Frontiers in Plant …, 2021 - frontiersin.org
CRISPR-Cas genome editing technology is developing at a rapid pace and new molecular
tools, such as CRISPR nucleases, are becoming regularly available. As part of this …
tools, such as CRISPR nucleases, are becoming regularly available. As part of this …
Cas9-NG greatly expands the targeting scope of the genome-editing toolkit by recognizing NG and other atypical PAMs in rice
B Ren, L Liu, S Li, Y Kuang, J Wang, D Zhang, X Zhou… - Molecular plant, 2019 - cell.com
CRISPR technologies enabling precise genome manipulation are valuable for gene function
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …
studies and molecular crop breeding. However, the requirement of a protospacer adjacent …
Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease
T Lawrenson, O Shorinola, N Stacey, C Li… - Genome biology, 2015 - Springer
Abstract Background The RNA-guided Cas9 system represents a flexible approach for
genome editing in plants. This method can create specific mutations that knock-out or alter …
genome editing in plants. This method can create specific mutations that knock-out or alter …
Targeted gene manipulation in plants using the CRISPR/Cas technology
D Zhang, Z Li, JF Li - Journal of Genetics and Genomics, 2016 - Elsevier
The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse
organisms including plants, and has quickly evolved into a suite of versatile tools for …
organisms including plants, and has quickly evolved into a suite of versatile tools for …
The CRISPR/Cas9 system and its applications in crop genome editing
A Bao, DJ Burritt, H Chen, X Zhou, D Cao… - Critical reviews in …, 2019 - Taylor & Francis
The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR
associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 …
associated protein9) system is an RNA-guided genome editing tool that consists of a Cas9 …
Optimizing ErCas12a for efficient gene editing in Arabidopsis thaliana
J Pietralla, N Capdeville, P Schindele… - Plant Biotechnology …, 2024 - Wiley Online Library
Summary The Er Cas12a nuclease, also known as MAD7, is part of a CRISPR/Cas system
from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31 …
from Eubacterium rectale and distantly related to Cas12a nucleases. As it shares only 31 …
Genome-wide prediction of highly specific guide RNA spacers for CRISPR–Cas9-mediated genome editing in model plants and major crops
K Xie, J Zhang, Y Yang - Molecular plant, 2014 - cell.com
Dear Editor, RNA-guided genome editing (RGE) using the Streptococcus pyogenes CRISPR–
Cas9 system (Jinek et al., 2012; Cong et al., 2013; Mali et al., 2013b) is emerging as a …
Cas9 system (Jinek et al., 2012; Cong et al., 2013; Mali et al., 2013b) is emerging as a …
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
Hs1Cas12a and Ev1Cas12a confer efficient genome editing in plants
Cas12a, also known as Cpf1, is a highly versatile CRISPR-Cas enzyme that has been
widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a has unique …
widely used in genome editing. Unlike its well-known counterpart, Cas9, Cas12a has unique …
A quick guide to CRISPR sgRNA design tools
VA Brazelton Jr, S Zarecor, DA Wright, Y Wang… - GM crops & …, 2015 - Taylor & Francis
Targeted genome editing is now possible in nearly any organism and is widely
acknowledged as a biotech game-changer. Among available gene editing techniques, the …
acknowledged as a biotech game-changer. Among available gene editing techniques, the …