[HTML][HTML] SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds
We describe here a CRISPR simultaneous and wide-editing induced by a single system
(SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding …
(SWISS), in which RNA aptamers engineered in crRNA scaffold recruit their cognate binding …
[HTML][HTML] CRISPR/Cas9 platforms for genome editing in plants: developments and applications
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …
[HTML][HTML] Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes
Substantial efforts are being made to optimize the CRISPR/Cas9 system for precision crop
breeding. The avoidance of transgene integration and reduction of off-target mutations are …
breeding. The avoidance of transgene integration and reduction of off-target mutations are …
Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins
This protocol is an extension to: Nat. Protoc. 9, 2395–2410 (2014); doi: 10.1038/nprot.
2014.157; published online 18 September 2014 In recent years, CRISPR/Cas9 has …
2014.157; published online 18 September 2014 In recent years, CRISPR/Cas9 has …
Targeted large fragment deletion in plants using paired crRNAs with type I CRISPR system
Y Li, B Huang, J Chen, L Huang, J Xu… - Plant Biotechnology …, 2023 - Wiley Online Library
The CRISPR‐Cas systems have been widely used as genome editing tools, with type II and
V systems typically introducing small indels, and type I system mediating long‐range …
V systems typically introducing small indels, and type I system mediating long‐range …
Editing plant genomes with CRISPR/Cas9
K Belhaj, A Chaparro-Garcia, S Kamoun… - Current opinion in …, 2015 - Elsevier
Highlights•Cas9 is an RNA-guided DNA endonuclease innate to prokaryotic immune
systems.•CRISPR/Cas9 has recently emerged as a powerful genome editing …
systems.•CRISPR/Cas9 has recently emerged as a powerful genome editing …
[HTML][HTML] Grand challenges in genome editing in plants
B Yang - Frontiers in Genome Editing, 2020 - frontiersin.org
The world depends on agriculture, and agriculture itself faces grand challenges, such as
meeting the needs for food, fiber, and bioenergy required by the continuously increasing …
meeting the needs for food, fiber, and bioenergy required by the continuously increasing …
The CRISPR-Cas9 technology: closer to the ultimate toolkit for targeted genome editing
F Quétier - Plant Science, 2016 - Elsevier
The first period of plant genome editing was based on Agrobacterium; chemical
mutagenesis by EMS (ethyl methanesulfonate) and ionizing radiations; each of these …
mutagenesis by EMS (ethyl methanesulfonate) and ionizing radiations; each of these …
Targeted mutagenesis in Arabidopsis thaliana using CRISPR‐Cas12b/C2c1
F Wu, X Qiao, Y Zhao, Z Zhang, Y Gao… - Journal of Integrative …, 2020 - Wiley Online Library
Abstract Cas12b/C2c1 is a newly identified class 2 CRISPR endonuclease that was recently
engineered for targeted genome editing in mammals and rice. To explore the potential …
engineered for targeted genome editing in mammals and rice. To explore the potential …
[HTML][HTML] Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis
Bacterial CRISPR systems have been widely adopted to create operator-specified site-
specific nucleases. Such nuclease action commonly results in loss-of-function alleles …
specific nucleases. Such nuclease action commonly results in loss-of-function alleles …