Biological processes involving movement, such as muscle contraction or the opening of an
ion channel through a membrane, are mediated through conformational changes. These …

Cryo-electron microscopy (cryo-EM) has for a long time been a technique of choice for
determining structure of large and flexible macromolecular complexes that were difficult to …

Single particle cryo-electron microscopy (cryo-EM) is a technique aimed at structure
determination of large macromolecular complexes in their unconstrained, physiological …

The invention of the electron microscope has greatly enhanced the view scientists have of
small structural details. Since its implementation, this technology has undergone …

Cryo‐electron microscopy (cryo‐EM) is increasingly becoming a mainstream technology for
studying the architecture of cells, viruses and protein assemblies at molecular resolution …

Structure-based drug design plays a central role in therapeutic development. Until recently,
protein crystallography and NMR have dominated experimental approaches to obtain …

Background Since the introduction of what became today's standard for cryo-embedding of
biological macromolecules at native conditions more than 30 years ago, techniques and …

Highlights•Recent cryo-EM technology allows near-atomic structural resolution of many
complexes.•Conformational changes, functionally relevant, hinder higher resolution of …

Structural biology is the study of the molecular architecture of proteins and nucleic acids,
which are the basis for all life forms. Structural biology came into its own as a field during the …

Abstract Knowledge of the three-dimensional structures of proteins and other biological
macromolecules often aids understanding of how they perform complicated tasks in the cell …