Cryo-electron microscopy reconstruction methods are uniquely able to reveal structures of
many important macromolecules and macromolecular complexes. EMDataBank. org, a joint …

Cryogenic-electron microscopy (cryo-EM) has recently emerged as a powerful technique in
structural biology that is capable of delivering high-resolution density maps of …

Recent advances in the field of electron cryomicroscopy (cryo-EM) have resulted in a rapidly
increasing number of atomic models of biomacromolecules that have been solved using this …

For many years, structure determination of biological macromolecules by cryo-electron
microscopy (cryo-EM) was limited to large complexes or low-resolution models. With recent …

Highlights•CX-MS identifies protein–protein interactions.•Cross-linking strategies targeting
different amino acids are available.•Combining CX-MS and cryo-EM delivers high-resolution …

Single-particle cryo electron microscopy (cryoEM) is a technique for determining three-
dimensional (3D) structures from projection images of molecular complexes preserved in …

Structural biology is the study of the molecular architecture of proteins and nucleic acids,
which are the basis for all life forms. Structural biology came into its own as a field during the …

Cryo-electron microscopy (cryo-EM) is a structural molecular and cellular biology technique
that has experienced major advances in recent years. Technological developments in image …

For many macromolecular assemblies, both a cryo-electron microscopy map and atomic
structures of its component proteins are available. Here we describe a method for fitting and …

Highlights•Near-atomic resolution cryo-EM structures of challenging targets can now be
obtained.•Advances in sample preparation increases chance of high-resolution structure …