Kinetic basis for DNA target specificity of CRISPR-Cas12a
Class 2 CRISPR-Cas nucleases are programmable genome editing tools with promising
applications in human health and disease. However, DNA cleavage at off-target sites that …
applications in human health and disease. However, DNA cleavage at off-target sites that …
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Abstract Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided
genome editing and transcription regulation in eukaryotic cells, yet their in vivo target …
genome editing and transcription regulation in eukaryotic cells, yet their in vivo target …
PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
T Karvelis, G Bigelyte, JK Young, Z Hou… - Nucleic acids …, 2020 - academic.oup.com
In recent years, CRISPR-associated (Cas) nucleases have revolutionized the genome
editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a …
editing field. Being guided by an RNA to cleave double-stranded (ds) DNA targets near a …
Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
Cas12a (also called Cpf1) is a representative type VA CRISPR effector RNA-guided DNA
endonuclease, which provides an alternative to type II CRISPR–Cas9 for genome editing …
endonuclease, which provides an alternative to type II CRISPR–Cas9 for genome editing …
RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?
G Gasiunas, V Siksnys - Trends in Microbiology, 2013 - cell.com
Tailor-made nucleases for precise genome modification, such as zinc finger or TALE
nucleases, currently represent the state-of-the-art for genome editing. These nucleases …
nucleases, currently represent the state-of-the-art for genome editing. These nucleases …
Cut site selection by the two nuclease domains of the Cas9 RNA-guided endonuclease
H Chen, J Choi, S Bailey - Journal of Biological Chemistry, 2014 - ASBMB
Cas9, the RNA-guided DNA endonuclease from the CRISPR-Cas (clustered regularly
interspaced short palindromic repeat–CRISPR-associated) system, has been adapted for …
interspaced short palindromic repeat–CRISPR-associated) system, has been adapted for …
Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
CRISPR/Cas9 is a programmable genome editing tool widely used for biological
applications and engineered Cas9s have increased discrimination against off-target …
applications and engineered Cas9s have increased discrimination against off-target …
An alpha-helical lid guides the target DNA toward catalysis in CRISPR-Cas12a
CRISPR-Cas12a is a powerful RNA-guided genome-editing system that generates double-
strand DNA breaks using its single RuvC nuclease domain by a sequential mechanism in …
strand DNA breaks using its single RuvC nuclease domain by a sequential mechanism in …
Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
F Chen, X Ding, Y Feng, T Seebeck, Y Jiang… - Nature …, 2017 - nature.com
Bacterial CRISPR–Cas systems comprise diverse effector endonucleases with different
targeting ranges, specificities and enzymatic properties, but many of them are inactive in …
targeting ranges, specificities and enzymatic properties, but many of them are inactive in …
CRISPR-Cas orthologues and variants: optimizing the repertoire, specificity and delivery of genome engineering tools
A Cebrian-Serrano, B Davies - Mammalian Genome, 2017 - Springer
Robust and cost-effective genome editing in a diverse array of cells and model organisms is
now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas …
now possible thanks to the discovery of the RNA-guided endonucleases of the CRISPR-Cas …