Tn5 transposase (Tnp) binds to Tn5 and IS50 end inverted repeats, the outside end (OE)
and the inside end (IE), to initiate transposition. We report the isolation of four Tnp mutants …

Five single base pair mutations that increase expression of the tnpn (transposase) gene of
the Tn3 transposon approximately 30-fold, but which still allow the gene to be regulated …

Conjugative transposition of transposon Tn916 has been shown to proceed by excision of
the transposon in the donor strain and insertion of this element in the recipient. This process …

A Lac+ papillation assay was used to identify mutants (tex) of Escherichia coli that exhibit an
increased frequency of precise excision of a lacZ:: Tn10dKan insertion. Three tex strains had …

The Tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing
Escherichia coli strain harboring a transposase overproducer plasmid. This accumulation of …

Integration host factor (IHF) is a DNA-binding and-bending protein that has been found in a
number of gram-negative bacteria. Here we describe the cloning, sequencing, and …

Summary 247 independent events involving insertion of the Tn3 transposable element into a
4 kb constructed plasmid (pTU4) of partially known DNA sequence were studied by …

It has been reported that transposition of Tn 3 is temperature-sensitive. The effect of
temperature on the transposition of other class II bacterial transposable elements is reported …

The complete nucleotide sequence of the Staphylococcus aureus transposon Tn554, which
encodes resistance to erythromycin and spectinomycin, was determined by the dideoxy …

We have identified the Escherichia coli RNA polymerase-binding sites and the transcription
initiation sites in the transposon Tn3. Results from nitrocellulose filter-binding assays …