The unique identity of each cell is the result of differential gene expression. A new strategy
for differential cDNA screening introduced by Liang and Pardee utilizes anchored oligo-dT …

Progress toward complete sequencing of all human genes through the Human Genome
Project has already resulted in a need for methods that allow quantitative expression …

Recent advances in molecular biology have led to development of methods to evaluate the
in vivo expression of specific genes in epithelial cells of the human respiratory tract. These …

The identification of genetic factors important in lung development and function will help in
understanding the underlying molecular mechanisms of respiratory disease …

A blood assay for detection of lung cancer biomarkers could significantly improve cancer
patient prognosis and survival rates. Amplified fragment length polymorphism‐differential …

The lung is composed of a complex assemblage of more than 40 different cell types.
Therefore, investigative techniques that rely on samples derived from whole lung …

Fibroblasts are the major cell type responsible for synthesizing matrix constituents in lung
and other connective tissues. Evidence indicates that fibroblasts are heterogeneous, and …

The human genome has been completely sequenced. The development of innovative
methodologies and tools to understand the functions of human genes in health and disease …

A bank of surgically resected human lung tissues frozen at− 70° C after being inflated with
support medium for cutting frozen tissue and a separate group inflated with fixative and …

Publisher Summary This chapter describes the protocols for fluorescent differential display
(FDD), using an automated DNA sequencer and a fluorescent image analyzer. It presents …