Infection of vertebrate cells with alphaviruses normally leads to prodigious expression of
virus-encoded genes and a dramatic inhibition of host protein synthesis. Recombinant …

Several DNA-based Sindbis virus vectors were constructed to investigate the feasibility and
potential applications for initiating the virus life cycle in cells transfected directly with plasmid …

Since the recovery of infectious RNA transcripts from full-length cDNA clones, alphavirus
genome RNAs have been engineered to allow expression of heterologous RNAs and …

Alphavirus replicon vectors are well suited for applications where transient, high-level
expression of a heterologous gene is required. Replicon vector expression in cells leads to …

Alphaviruses are a well-characterized group of positive-strand RNA viruses. The
identification of cis-acting elements in their genomes and their replication strategy have …

Genetically engineered RNA transcripts coding for various Sindbis virus (SIN) genes were
used to study structure and sequence requirements of RNA recombination in BHK cells …

We constructed full-length cDNA clones of Sindbis virus that can be transcribed in vitro by
SP6 RNA polymerase to produce infectious genome-length transcripts. Viruses produced …

The direct Intramuscular delivery of naked plasmld DNA has been demonstrated to allow
expression of encoded heterologous genes in the target myocytes. The method has been …

Sindbis virus, an enveloped virus with a single-stranded RNA genome, was engineered to
express a bacterial protein, chloramphenicol acetyltransferase (CAT), in cultured insect …

BACKGROUND OF INVENTION Advances in recombinant DNA technology and ge netic
engineering have provided means for producing in bacteria specific proteins of commercial …