Expressed protein ligation is a simple and powerful method in protein engineering to
introduce sequences of unnatural amino acids, posttranslational modifications, and …

Our knowledge of biological systems relies increasingly on the ability of quantifying and
imaging intracellular signals and events in living subjects. The development of novel …

Protein splicing performed by inteins provides powerful opportunities to manipulate protein
structure and function, however, detailed mechanistic knowledge of the multistep pathway to …

Protein trans-splicing (PTS) exerted by split inteins is a protein ligation reaction which
enables overcoming the barriers of conventional heterologous protein production. We …

Laboratory-created small-molecule-dependent inteins enable protein structure and function
to be controlled posttranslationally in living cells. Previously we evolved inteins that splice …

The protein trans-splicing (PTS) activity of naturally split inteins has found widespread use in
chemical biology and biotechnology. However, currently used naturally split inteins suffer …

Inteins are proteins that excise themselves out of host proteins and ligate the flanking
polypeptides in an auto‐catalytic process called protein splicing. In nature, inteins are either …

Protein splicing is a self‐catalyzed process in which an internal protein domain (the intein) is
excised from its flanking sequences, linking them together with a canonical peptide bond …

Inteins are unique single-turnover enzymes that can excise themselves from the precursor
protein without the aid of any external cofactors or energy. In most cases, inteins are …