Obtaining insights into the molecular structure and dynamics of a protein by NMR
spectroscopy and other in-solution biophysical methods relies heavily on the incorporation …

Protein splicing is a posttranslational processing event that releases an internal protein
sequence from a protein precursor. During the splicing process the internal protein …

Protein-splicing inteins are widespread in nature and have found many applications in
protein research and engineering. The mechanism of protein splicing typically requires a …

Naturally split inteins mediate a traceless protein ligation process known as protein trans‐
splicing (PTS). Although frequently used in protein engineering applications, the efficiency of …

The discovery of inteins, which are protein-splicing elements, has stimulated interest for
various applications in chemical biology, bioseparations, drug delivery, and sensor …

Protein trans-splicing by split inteins holds great potential for the chemical modification and
semisynthesis of proteins. However, the structural requirements of the extein sequences …

Protein splicing is a posttranslational modification where intervening proteins (inteins)
cleave themselves from larger precursor proteins and ligate their flanking polypeptides …

Split inteins play an important role in modern protein semisynthesis techniques. These
naturally occurring protein splicing domains can be used for in vitro and in vivo protein …

Split intein-mediated protein trans-splicing has found extensive applications in chemical
biology, protein chemistry, and biotechnology. However, an enduring limitation of all well …

The success of genome sequencing has heightened the demand for new means to
manipulate proteins. An especially desirable goal is the ability to modify a target protein at a …