Passenger strand loading in overexpression experiments using microRNA mimics
MicroRNAs (miRNAs) are important regulators of gene function and manipulation of miRNAs
is a central component of basic research. Modulation of gene expression by miRNA gain-of …
is a central component of basic research. Modulation of gene expression by miRNA gain-of …
[HTML][HTML] Transfection of microRNA mimics should be used with caution
HY Jin, A Gonzalez-Martin, AV Miletic, M Lai… - Frontiers in …, 2015 - frontiersin.org
Transient transfection of chemically synthesized microRNA (miRNA) mimics is being used
extensively to study the functions and mechanisms of endogenous miRNAs. However, it …
extensively to study the functions and mechanisms of endogenous miRNAs. However, it …
Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs
Transfection of small RNAs (such as small interfering RNAs (siRNAs) and microRNAs
(miRNAs)) into cells typically lowers expression of many genes. Unexpectedly, increased …
(miRNAs)) into cells typically lowers expression of many genes. Unexpectedly, increased …
[HTML][HTML] Target gene expression levels and competition between transfected and endogenous microRNAs are strong confounding factors in microRNA high …
Background MicroRNA (miRNA) target genes tend to have relatively long and conserved
3'untranslated regions (UTRs), but to what degree these characteristics contribute to miRNA …
3'untranslated regions (UTRs), but to what degree these characteristics contribute to miRNA …
[HTML][HTML] On measuring miRNAs after transient transfection of mimics or antisense inhibitors
The ability to alter microRNA (miRNA) abundance is crucial for studying miRNA function. To
achieve this there is widespread use of both exogenous double-stranded miRNA mimics for …
achieve this there is widespread use of both exogenous double-stranded miRNA mimics for …
[HTML][HTML] MicroRNA target identification—experimental approaches
A Martinez-Sanchez, CL Murphy - Biology, 2013 - mdpi.com
MicroRNAs (miRNAs) are small non-coding RNA molecules of 21–23 nucleotides that
control gene expression at the post-transcriptional level. They have been shown to play a …
control gene expression at the post-transcriptional level. They have been shown to play a …
MicroRNA screening and the quest for biologically relevant targets
A Eulalio, M Mano - Journal of biomolecular screening, 2015 - journals.sagepub.com
MicroRNAs (miRNAs) are a class of genome-encoded small RNAs that post-transcriptionally
regulate gene expression by repressing target transcripts containing partially or fully …
regulate gene expression by repressing target transcripts containing partially or fully …
[HTML][HTML] Up-regulation of microRNA* strands by their target transcripts
During microRNA (miRNA) biogenesis, one strand of a 21–23 nucleotide RNA duplex is
preferentially selected for entry into an RNA-induced silencing complex (RISC). The other …
preferentially selected for entry into an RNA-induced silencing complex (RISC). The other …
The microrna
SY Ying, DC Chang, SL Lin - MicroRNA Protocols, 2013 - Springer
Abstract MicroRNAs (miRNAs), widely distributed, small regulatory RNA genes, target both
messenger RNA (mRNA) degradation and suppression of protein translation based on …
messenger RNA (mRNA) degradation and suppression of protein translation based on …
Experimental miRNA target validation
J Heyn, LC Hinske, C Ledderose, E Limbeck… - MicroRNA Protocols, 2013 - Springer
In the recent past, microRNAs (miRNAs) have gained significant attention as potent
regulators of gene expression. These small noncoding RNA molecules are currently of …
regulators of gene expression. These small noncoding RNA molecules are currently of …