Shotgun proteomics is a powerful analytic method to characterize complex protein mixtures
in combination with multidimensional liquid chromatography‐tandem mass spectrometry …

Hepatocytes are known to express a large number of characteristic proteins. Transformed
and cultured hepatocytes only partially maintain functional cell differentiation characteristics …

Hepatocellular carcinoma (HCC) is one of the leading causes of mortality from solid organ
malignancy worldwide. Because of the complexity of proteins within liver cells and tissues …

Freshly isolated human hepatocytes are considered the gold standard for in vitro studies of
liver functions, including drug transport, metabolism, and toxicity. For accurate predictions of …

Although mass spectrometry-based plasma proteomics enables sensitive and large-scale
discovery and validation of biomarkers for various diseases, its integrative application to …

The extraordinary developments made in the past decade in proteomic technologies, in
particular in mass spectrometry, have enabled investigators to consider designing studies to …

Abstract Background & Aims The human liver functions through a complex interplay
between parenchymal and non‐parenchymal cells. Mass spectrometry‐based proteomic …

In-gel digestion is commonly used after proteins are resolved by polyacrylamide gel
electrophoresis (SDS-PAGE, 2-DE). It can also be used on its own in conjunction with …

Parenchymatous organs consist of multiple cell types, primarily defined as parenchymal
cells (PCs) and nonparenchymal cells (NPCs). The cellular characteristics of these organs …

Proteomics has evolved into a powerful and widely used bioanalytical technique in the study
of cancer, especially hepatocellular carcinoma (HCC). In this review, we provide an up to …