Split inteins carry out a naturally occurring process known as protein trans‐splicing, where
two protein fragments bind to form a catalytically competent enzyme, then catalyze their own …

Split inteins are a class of naturally occurring proteins that carry out protein splicing in trans.
The chemical mechanism of protein trans-splicing is well-understood and has been …

Protein trans-splicing by split inteins has many uses in protein production and research.
Splicing proteins with synthetic peptides, which employs atypical split inteins, is particularly …

Protein splicing in trans by split inteins has increasingly become a powerful protein-
engineering tool for protein ligation, both in vivo and in vitro. Over 100 naturally occurring …

Protein trans-splicing (PTS) exerted by split inteins is a protein ligation reaction which
enables overcoming the barriers of conventional heterologous protein production. We …

Inteins are protein-intervening sequences that can self-excise and concomitantly splice
together the flanking polypeptides. Two-piece split inteins capable of protein trans-splicing …

Split intein-mediated protein trans-splicing has found extensive applications in chemical
biology, protein chemistry, and biotechnology. However, an enduring limitation of all well …

Protein trans‐splicing catalyzed by split inteins is a powerful technique for assembling a
polypeptide backbone from two separate parts. However, split inteins with robust efficiencies …

Split inteins associate to trigger protein splicing in trans, a post-translational modification in
which protein sequences fused to the intein pair are ligated together in a traceless manner …

Inteins catalyze a protein splicing reaction to excise the intein from a precursor protein and
join the flanking sequences (exteins) with a peptide bond. In a split intein, the intein …