Protein trans-splicing mediated by a split intein reconstitutes a protein backbone from two
parts. This virtually traceless autoprocessive reaction provides the basis for numerous …

Protein trans‐splicing catalyzed by split inteins has increasingly become useful as a protein
engineering tool. We solved the 1.0 Å‐resolution crystal structure of a fused variant from the …

Inteins are internal protein sequences that post-translationally self-excise and splice
together the flanking sequences, the so-called exteins. Natural and engineered inteins have …

Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology
and biotechnology. Herein, we describe the use of a consensus design approach to …

Naturally split inteins mediate a traceless protein ligation process known as protein trans‐
splicing (PTS). Although frequently used in protein engineering applications, the efficiency of …

Split inteins play an important role in modern protein semisynthesis techniques. These
naturally occurring protein splicing domains can be used for in vitro and in vivo protein …

Split inteins are parasitic genetic elements frequently found inserted into reading frames of
essential proteins. Their association and excision restore host protein function through a …

A split‐intein consists of two complementary fragments (N‐intein and C‐intein) that can
associate to carry out protein trans‐splicing. The Ssp GyrB S11 split‐intein is an engineered …

Split intein-mediated protein trans-splicing (PTS) is widely applied in chemical biology and
biotechnology to carry out traceless and specific protein ligation. However, the external …

Inteins are protein segments that are capable of enabling the ligation of flanking extein into a
new protein, a process known as protein splicing. Since its discovery, inteins have become …