▪ Abstract Intein is the protein equivalent of intron and has been discovered in increasing
numbers of organisms and host proteins. A self-splicing intein catalyzes its own removal …

Inteins are internal protein elements that self-excise from their host protein and catalyze
ligation of the flanking sequences (exteins) with a peptide bond. They are found in …

Protein splicing in trans results in the ligation of two protein or peptide segments linked to
appropriate intein fragments. We have characterized the trans‐splicing reaction mediated by …

Protein splicing is a post-translational reaction facilitated by an intein, or intervening protein,
which involves the removal of the intein and the ligation of the flanking polypeptides, or …

Intein-mediated protein splicing is a naturally occurring selfprocessing event in which the
intervening intein sequence is removed from a precursor protein and the flanking extein …

Protein splicing is a posttranslational process in which an intein segment excises itself from
two flanking peptides, referred to as exteins. In the native context, protein splicing results in …

Expressed protein ligation is a simple and powerful method in protein engineering to
introduce sequences of unnatural amino acids, posttranslational modifications, and …

Inteins catalyze a post-translational modification known as protein splicing, where the intein
removes itself from a precursor protein and concomitantly ligates the flanking protein …

Inteins are protein splicing elements that employ standard enzyme strategies to excise
themselves from precursor proteins and ligate the surrounding sequences (exteins). The …

Protein splicing is a robust multistep posttranslational process catalyzed by inteins. In the
Mtu RecA intein, a conserved block-F aspartate (D422) coordinates different steps in protein …