Rapid Screening and Identification of Methicillin-Resistant Staphylococcus aureus from Clinical Samples by Selective-Broth and Real-Time PCR Assay

H Fang, G Hedin - Journal of clinical microbiology, 2003 - Am Soc Microbiol
ABSTRACT A screening method for methicillin-resistant Staphylococcus aureus (MRSA) by
using selective broth and real-time PCR (broth-PCR) was developed and evaluated. The …

Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR

AJ Grisold, E Leitner, G Mühlbauer… - Journal of clinical …, 2002 - Am Soc Microbiol
ABSTRACT A molecular assay for the simultaneous detection of a Staphylococcus aureus-
specific gene and the mecA gene, responsible for the resistance to methicillin in …

Comparison of the Velogene Rapid MRSA identification assay, Denka MRSA-Screen assay, and BBL Crystal MRSA ID system for rapid identification of methicillin …

J Arbique, K Forward, D Haldane… - … microbiology and infectious …, 2001 - Elsevier
Early detection of methicillin-resistant S. aureus (MRSA) is critical for both the management
of infected patients, and the timely institution of appropriate infection control measures …

Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs

D Jonas, M Speck, FD Daschner… - Journal of Clinical …, 2002 - Am Soc Microbiol
ABSTRACT A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA),
obviating the need for subculture on agar media, was investigated. The combination of …

Multiplex PCR for Simultaneous Identification ofStaphylococcus aureus and Detection of Methicillin and Mupirocin Resistance

E Perez-Roth, F Claverie-Martın, J Villar… - Journal of clinical …, 2001 - Am Soc Microbiol
In this work, we describe a multiplex PCR assay for the detection of clinically relevant
antibiotic resistance genes harbored by some Staphylococcus aureus isolates and for the …

Development and evaluation of a PCR-based immunoassay for the rapid detection of methicillin-resistant Staphylococcus aureus

KJ Towner, DCS Talbot, R Curran… - Journal of medical …, 1998 - microbiologyresearch.org
A multiplex polymerase chain reaction (PCR), involving detection of the mecA and femB
genes, was combined with a novel immunoassay system capable of detecting specific PCR …

Evaluation of the IDI-MRSA Assay for Detection of Methicillin-Resistant Staphylococcus aureus from Nasal and Rectal Specimens Pooled in a Selective Broth

M Desjardins, C Guibord, B Lalonde… - Journal of Clinical …, 2006 - Am Soc Microbiol
Rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by PCR can be
performed directly from nasal specimens with the IDI-MRSA assay. To improve the efficiency …

Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) in Specimens from Various Body Sites: Performance Characteristics of the BD GeneOhm MRSA …

M Hombach, GE Pfyffer, M Roos… - Journal of clinical …, 2010 - Am Soc Microbiol
Universal surveillance upon patient admission is important in reducing the transmission of
methicillin-resistant Staphylococcus aureus (MRSA) and associated disease in hospitals …

Evaluation of Three Rapid Methods for Detection of Methicillin Resistance in Staphylococcus aureus

L Louie, SO Matsumura, E Choi, M Louie… - Journal of clinical …, 2000 - Am Soc Microbiol
ABSTRACT The probe-based Velogene Rapid MRSA Identification Assay (ID Biomedical
Corp., Vancouver, British Columbia, Canada) and the latex agglutination MRSA-Screen …

Rapid Detection of Methicillin-Resistant Staphylococcus aureus Directly from Sterile or Nonsterile Clinical Samples by a New Molecular Assay

P Francois, D Pittet, M Bento, B Pepey… - Journal of Clinical …, 2003 - Am Soc Microbiol
ABSTRACT A rapid procedure was developed for detection and identification of methicillin-
resistant Staphylococcus aureus (MRSA) directly from sterile sites or mixed flora samples …