Class 2 CRISPR systems are exceptionally diverse, nevertheless, all share a single effector
protein that contains a conserved RuvC-like nuclease domain. Interestingly, the size of these …

Cas9, the RNA-guided DNA endonuclease from the CRISPR-Cas (clustered regularly
interspaced short palindromic repeat–CRISPR-associated) system, has been adapted for …

DNA-targeting CRISPR-Cas systems, such as those employing the RNA-guided Cas9 or
Cas12 endonucleases, have revolutionized our ability to predictably edit genomes and …

CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …

Deployment of RNA-guided DNA endonuclease CRISPR–Cas technology has led to radical
advances in biology. As the functional diversity of CRISPR–Cas and parallel systems is …

CRISPR-Cas12c/d proteins share limited homology with Cas12a and Cas9 bacterial
CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA …

CRISPR–Cas is a bacterial defense system against phage infection and nucleic acid
invasion. Class 2 type II CRISPR–Cas9 has also been widely used for genome engineering …

The continued expansion of the genome-editing toolbox necessitates methods to
characterize important properties of CRISPR–Cas enzymes. One such property is the …

Summary CRISPR-Cas12a (Cpf1), a type V CRISPR-associated nuclease, provides
bacterial immunity against bacteriophages and plasmids but also serves as a tool for …

Editorial summary CRISPR–Cas systems have provided revolutionary tools for genome
editing. The discovery of Cas proteins with alternative activities has now enabled sensitive …