Abstract CRISPR-Cas9 and CRISPR-Cpf1 systems have been successfully harnessed for
genome editing. In the CRISPR-Cas9 system, the preordered A-form RNA seed sequence …

Microbial CRISPR-Cas systems are divided into Class 1, with multisubunit effector
complexes, and Class 2, with single protein effectors. Currently, only two Class 2 effectors …

CRISPR-Cas systems are host-encoded pathways that protect microbes from viral infection
using an adaptive RNA-guided mechanism. Using genome-resolved metagenomics, we find …

Development of tools for targeted genome editing and regulation of gene expression has
significantly expanded our ability to elucidate the mechanisms of interesting biological …

CRISPR-Cas constitutes the adaptive immune system of bacteria and archaea. This RNA-
mediated sequence-specific recognition and targeting machinery has been used broadly for …

Since the rapid emergence of clustered regulatory interspaced short palindromic repeats
(CRISPR)-CRISPR-associated protein 9 (Cas9) system, developed as a genome …

Targeted gene regulation on a genome-wide scale is a powerful strategy for interrogating,
perturbing, and engineering cellular systems. Here, we develop a method for controlling …

The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …

CRISPR-Cas12a (Cpf1) proteins are RNA-guided enzymes that bind and cut DNA as
components of bacterial adaptive immune systems. Like CRISPR-Cas9, Cas12a has been …

CRISPR (clustered regularly interspaced short palindromic repeats) utility relies on a stable
Cas effector complex binding to its target site. However, a Cas complex bound to DNA may …