As a complementary approach to two-dimensional polyacrylamide gel electrophoresis (2D-
PAGE), multi-dimensional chromatography separation methods have been widely applied in …

As a complementary approach to 2D-PAGE, multidimensional liquid chromatography
(MDLC) separation methods have been widely applied in all kinds of biological sample …

Proteome was first coined by describing the set of proteins encoded by the genome, and the
study of proteome, named as proteomics, aims at globally characterizing all components in a …

Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …

Current solution based proteomic analysis methods are generally based on enzymatic
digestion of a protein mixture followed by separation using multidimensional liquid …

Column liquid chromatography (LC) increasingly is used as a complement to or replacement
for gel electrophoresis, but the coupling of two or more chromatographic modes generally is …

Large-scale proteomics often employs two orthogonal separation methods to fractionate
complex peptide mixtures. Fractionation can involve ion exchange separation coupled to …

Proteomics technologies demonstrate enormous data-gathering capabilities to discover
disease-specific biomarkers in serum, plasma, urine, tissue and other biological samples …

In this paper, we present a combined top-down/bottom-up proteomic analysis workflow for
the characterization of proteomic samples. This workflow combines protein fractionation …

Multidimensional liquid-based separation techniques are described for maximizing the
resolution of the enormous number of peptides generated upon tryptic digestion of …