Recent development of multi-dimensional chromatography strategies in proteome research
As a complementary approach to two-dimensional polyacrylamide gel electrophoresis (2D-
PAGE), multi-dimensional chromatography separation methods have been widely applied in …
PAGE), multi-dimensional chromatography separation methods have been widely applied in …
Development of multidimensional liquid chromatography and application in proteomic analysis
M Gao, D Qi, P Zhang, C Deng… - Expert Review of …, 2010 - Taylor & Francis
As a complementary approach to 2D-PAGE, multidimensional liquid chromatography
(MDLC) separation methods have been widely applied in all kinds of biological sample …
(MDLC) separation methods have been widely applied in all kinds of biological sample …
Recent advances in multidimensional separation for proteome analysis
H Yuan, B Jiang, B Zhao, L Zhang, Y Zhang - Analytical Chemistry, 2018 - ACS Publications
Proteome was first coined by describing the set of proteins encoded by the genome, and the
study of proteome, named as proteomics, aims at globally characterizing all components in a …
study of proteome, named as proteomics, aims at globally characterizing all components in a …
Multi-dimensional liquid chromatography in proteomics—a review
Proteomics is the large-scale study of proteins, particularly their expression, structures and
functions. This still-emerging combination of technologies aims to describe and characterize …
functions. This still-emerging combination of technologies aims to describe and characterize …
Multidimensional separation of peptides for effective proteomic analysis
HJ Issaq, KC Chan, GM Janini, TP Conrads… - … of Chromatography B, 2005 - Elsevier
Current solution based proteomic analysis methods are generally based on enzymatic
digestion of a protein mixture followed by separation using multidimensional liquid …
digestion of a protein mixture followed by separation using multidimensional liquid …
Multidimensional liquid chromatography in proteomic studies: most protein and peptide samples analyzed in proteomic studies tax the separation power of traditional …
T Wehr - LC-GC North America, 2002 - go.gale.com
Column liquid chromatography (LC) increasingly is used as a complement to or replacement
for gel electrophoresis, but the coupling of two or more chromatographic modes generally is …
for gel electrophoresis, but the coupling of two or more chromatographic modes generally is …
Off-line multidimensional liquid chromatography and auto sampling result in sample loss in LC/LC–MS/MS
S Magdeldin, JJ Moresco, T Yamamoto… - Journal of proteome …, 2014 - ACS Publications
Large-scale proteomics often employs two orthogonal separation methods to fractionate
complex peptide mixtures. Fractionation can involve ion exchange separation coupled to …
complex peptide mixtures. Fractionation can involve ion exchange separation coupled to …
Multidimensional protein identification technology for clinical proteomic analysis
P Mauri, M Scigelova - Clinical chemistry and laboratory medicine, 2009 - degruyter.com
Proteomics technologies demonstrate enormous data-gathering capabilities to discover
disease-specific biomarkers in serum, plasma, urine, tissue and other biological samples …
disease-specific biomarkers in serum, plasma, urine, tissue and other biological samples …
Integration of multidimensional chromatographic protein separations with a combined “top-down” and “bottom-up” proteomic strategy
In this paper, we present a combined top-down/bottom-up proteomic analysis workflow for
the characterization of proteomic samples. This workflow combines protein fractionation …
the characterization of proteomic samples. This workflow combines protein fractionation …
Highly efficient peptide separations in proteomics: Part 2: Bi-and multidimensional liquid-based separation techniques
Multidimensional liquid-based separation techniques are described for maximizing the
resolution of the enormous number of peptides generated upon tryptic digestion of …
resolution of the enormous number of peptides generated upon tryptic digestion of …