Flow cytometric determination of genome size in Pinus

IEW O'Brien, DR Smith, RC Gardner, BG Murray - Plant Science, 1996 - Elsevier
The nuclear 2C DNA content of seven Pinus species and Pseudotsuga menziesii was
determined by flow cytometric analysis. Conditions for nuclear isolation were adapted to …

Nuclear DNA content of Pinus sylvestris (L.) as determined by laser flow cytometry

JPT Valkonen, M Nygren, A Ylönen, L Mannonen - Genetica, 1994 - Springer
Nuclear DNA content was determined in nuclei isolated from needles, stems and roots of in
vitro grown seedlings and from megagametophytes and embryo of mature seeds in three …

Genome size and environmental factors in the genus Pinus

I Wakamiya, RJ Newton, JS Johnston… - American Journal of …, 1993 - Wiley Online Library
Nuclear 1C DNA content in haploid megagametophyte tissue of 18 North American and one
exotic Pinus species was determined using scanning microspectrophotometry. The nuclear …

INTRASPECIFIC VARIATION OF NUCLEAR DNA CONTENT IN PINUS RESINOSA AIT.

NK Dhir, JP Miksche - Canadian Journal of Genetics and …, 1974 - cdnsciencepub.com
Pinus resinosa displays a rather uniform phenotype over a relatively small geographic
distribution with a 9-degree latitudinal spread. DNA quantity per cell from 20 seed sources …

Genome size and base composition of five Pinus species from the Balkan region

F Bogunic, E Muratovic, SC Brown, S Siljak-Yakovlev - Plant Cell Reports, 2003 - Springer
The 2C DNA content and base composition of five Pinus (2 n= 24) species and two Pinus
subspecies from the Balkan region have been estimated by flow cytometry. P. heldreichii …

Chromosome identification and comparative karyotypic analyses of four Pinus species

M Hizume, F Shibata, Y Matsusaki… - Theoretical and Applied …, 2002 - Springer
Chromosomal landmarks in four Pinus species: P. densiflora, P. thunbergii, P. sylvestris, and
P. nigra were identified by fluorescence in situ hybridization (FISH) using hapten-or …

Plant genome size estimation by flow cytometry: inter-laboratory comparison

J Doležel, J Greilhuber, S Lucretti, A Meister… - Annals of …, 1998 - academic.oup.com
Flow cytometry is a convenient and rapid method that has been used extensively for
estimation of nuclear genome size in plants. In contrast to general expectations, results …

Optimizing conditions for DNA isolation fromPinus radiata

E Ostrowska, M Muralitharan, S Chandler… - In Vitro Cellular & …, 1998 - Springer
Genomic DNA was isolated from in vitro Pinus radiata seedling with five DNA isolation
protocols commonly used for pines. The methods described by Jobes et al.(1995) and …

Preparation of pea (Pisum sativum L.) chromosome and nucleus suspensions from single root tips

G Gualberti, J Doležel, J Macas, S Lucretti - Theoretical and Applied …, 1996 - Springer
A high-yield method for the isolation of intact nuclei and chromosomes in suspension from a
variable number of pea root tips (1–10) has been developed. This procedure is based on a …

[PDF][PDF] Estimating nuclear DNA content in peach and related diploid species using laser flow cytometry and DNA hybridization

WV Baird, AS Estager, JK Wells - Journal of the American Society …, 1994 - researchgate.net
Using laser flow cytometry, nuclear DNA amounts were estimated for 12 Prunus species,
representing three subgenera [Prunophora (Prunus), Amygdalus, and Cerasus …