Coupling sequences are the 6 bp flanking the conjugative transposon Tn916 and are
thought to play a role in determining the frequency of conjugative transposition. The affinity …

When a bacterial transposon jumps to a new genomic location, does it replicate itself in the
process thus generating two transposon copies, or does it excise itself from the old site and …

The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific
genomic integration in mammalian cells, recombining donor plasmids bearing the attB …

Acquisition of foreign DNA by natural transformation is an important mechanism of
adaptation and evolution in diverse microbial species. Here, we characterize the mechanism …

Phage lambda Integrase (Int) is the prototype of the so-called integrase family of
conservative site-specific recombinases, which includes Cre and FLP. The natural function …

Currently two site-specific recombinases are available for engineering the mouse genome:
Cre from P1 phage, and Flp from yeast,. Both enzymes catalyze recombination between two …

ΦC31 integrase is a member of the large serine subfamily and is required for the
recombination of the phage genome into the host chromosome, either to establish or exit …

Retroviral integrase is an enzyme responsible for the integration of retroviruses. A single
mutation in the integrase core domain can severely compromise its integration ability …

Conjugative transposons are transposable elements that can cause gram-positive bacteria
to be conjugational donors to promote antibiotic resistance. The can conjugate by moving …

The Synechocystis sp. PCC6803 insertion sequence ISY100 (ISTcSa) belongs to the
Tc1/mariner/IS630 family of transposable elements. ISY100 transposase was purified and …