Diagnóstico molecular de parasitosis intestinales

E Dacal, PC Köster, D Carmena - Enfermedades infecciosas y …, 2020 - Elsevier
Infections causes by parasites of the gastrointestinal tract are a global public health problem.
In industrialised countries, their particular epidemiological (low general prevalence of …

Application of PCR-based methods for diagnosis of intestinal parasitic infections in the clinical laboratory

JJ Verweij - Parasitology, 2014 - cambridge.org
For many years PCR-and other DNA-based methods of pathogen detection have been
available in most clinical microbiology laboratories; however, until recently these tools were …

Intestinal parasitic infections in an industrialized country; a new focus on children with better DNA-based diagnostics

JJ Verweij, L Van Lieshout - Parasitology, 2011 - cambridge.org
In recent years, the isolation of parasitic DNA from faecal samples and PCR techniques,
have been improved and simplified. Moreover, the introduction of real-time PCR has made it …

[HTML][HTML] Clinical consequences of new diagnostic tools for intestinal parasites

L Van Lieshout, M Roestenberg - Clinical Microbiology and Infection, 2015 - Elsevier
Following the success of nucleic acid-based detection in virology and bacteriology, multiplex
real-time PCRs are increasingly used as first-line diagnostics in clinical parasitology …

Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections

JJ Verweij, CR Stensvold - Clinical microbiology reviews, 2014 - Am Soc Microbiol
Over the past few decades, nucleic acid-based methods have been developed for the
diagnosis of intestinal parasitic infections. Advantages of nucleic acid-based methods are …

New technologies for detection of enteric parasites

U Ryan, A Paparini, C Oskam - Trends in parasitology, 2017 - cell.com
Enteric parasites are major contributors to the global diarrhoeal disease load, infecting>
67.2 million people. Their prevalence and clinical impact, however, are underestimated due …

Molecular biology can change the classic laboratory approach for intestinal protozoan infections

F Formenti, M Valerio, M Guerriero, F Perandin… - Frontiers in …, 2017 - frontiersin.org
For many years microscopy has been considered the mainstay of the diagnosis of parasitic
infections. In our laboratory, before the advent of molecular biology, the approach for the …

[HTML][HTML] Parasitological diagnosis combining an internally controlled real-time PCR assay for the detection of four protozoa in stool samples with a testing algorithm for …

LESB van Coppenraet, JA Wallinga, G Ruijs… - Clinical Microbiology …, 2009 - Elsevier
Molecular detection of gastrointestinal protozoa is more sensitive and more specific than
microscopy but, to date, has not routinely replaced time-consuming microscopic analysis …

[HTML][HTML] Comparison of three commercial multiplex PCR assays for the diagnosis of intestinal protozoa

B Autier, S Belaz, R Razakandrainibe, JP Gangneux… - Parasite, 2018 - ncbi.nlm.nih.gov
Although microscopic examination of stool samples remains the reference method for the
diagnosis of intestinal protozoal infections, these techniques are time-consuming and …

Clinical consequences of polymerase chain reaction‐based diagnosis of intestinal parasitic infections

LH Rijsman, JF Monkelbaan… - … of gastroenterology and …, 2016 - Wiley Online Library
The implementation of polymerase chain reaction (PCR)‐based diagnostics of intestinal
protozoa has led to higher sensitivity and (subtype) specificity, more convenient sampling …