Abstract 3D organotypic culture models such as organoids and multicellular tumor spheroids
(MCTS) are becoming more widely used for drug discovery and toxicology screening. As a …

3D cell cultures are being utilized for drug discovery and development. However, there are
still challenges to implementing them generally in quantitative high-throughput screening …

High content imaging (HCI) is a multiplexed cell staining assay developed for better
understanding of complex biological functions and mechanisms of drug action, and it has …

The introduction of more relevant cell models in early preclinical drug discovery, combined
with high-content imaging and automated analysis, is expected to increase the quality of …

Abstract Phenotypic or High Content Screening (HCS) is becoming more widely used for
primary screening campaigns in drug discovery. Currently the vast majority of HCS …

New cancer drug approval rates are≤ 5% despite significant investments in cancer
research, drug discovery and development. One strategy to improve the rate of success of …

Adoption of spheroids within high-content screening (HCS) has lagged behind high-
throughput screening (HTS) due to issues with running complex assays on large three …

Abstract Three-dimensional (3D) cell culture technology has been steadily studied since the
1990's due to its superior biocompatibility compared to the conventional two-dimensional …

Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

Introduction: High-resolution microscopy using fluorescent probes is a powerful tool to
investigate individual cell structure and function, cell subpopulations and mechanisms …