Overexpression of phospholipid: diacylglycerol acyltransferase in Brassica napus results in changes in lipid metabolism and oil accumulation

S Fenyk, HK Woodfield, TB Romsdahl… - Biochemical …, 2022 - portlandpress.com
The regulation of lipid metabolism in oil seeds is still not fully understood and increasing our
knowledge in this regard is of great economic, as well as intellectual, importance. Oilseed …

Transgenic manipulation of triacylglycerol biosynthetic enzymes in B. napus alters lipid-associated gene expression and lipid metabolism

P Liao, T Lechon, T Romsdahl, H Woodfield, S Fenyk… - Scientific reports, 2022 - nature.com
Oilseed rape (Brassica napus) is an important crop that is cultivated for the oil (mainly
triacylglycerol; TAG) it produces in its seeds. TAG synthesis is controlled mainly by key …

[PDF][PDF] Transformation of Brassica napus with diacylglycerol acyltransferase-1 results in increased seed oil content

RJ Weselake, S Shah, DC Taylor, J Zou… - Current advances in …, 2007 - researchgate.net
Abstract Diacylglycerol acyltransferase (DGAT, EC 2.3. 1.20) catalyzes the acyl-CoA-
dependent acylation of sn-1, 2-diacylglycerol (DAG) to generate triacylglycerol (TAG) in …

Alteration of the fatty acid composition of Brassica napus L. via overexpression of phospholipid: Diacylglycerol acyltransferase 1 from Sapium sebiferum (L.) Roxb.

B Zhou, W Fei, S Yang, F Yang, G Qu, W Tang, J Ou… - Plant Science, 2020 - Elsevier
Abstract Sapium sebiferum (L.) Roxb. plays an important role in traditional Chinese
medicine and is one of major woody oil tree in China. Phospholipid: diacylglycerol …

Two acyltransferases contribute differently to linolenic acid levels in seed oil

S Marmon, D Sturtevant, C Herrfurth… - Plant …, 2017 - academic.oup.com
Acyltransferases are key contributors to triacylglycerol (TAG) synthesis and, thus, are of
great importance for seed oil quality. The effects of increased or decreased expression of …

Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid: diacylglycerol transacylase gene (At5g13640)

V Mhaske, K Beldjilali, J Ohlrogge, M Pollard - Plant Physiology and …, 2005 - Elsevier
To more fully understand the function of phospholipid: diacylglycerol acyltransferase (PDAT,
EC 2.3. 1.158) in plants we have isolated and characterized a knockout mutant of …

Heterologous expression of two GPATs from Jatropha curcas alters seed oil levels in transgenic Arabidopsis thaliana

A Misra, K Khan, A Niranjan, V Kumar, VA Sane - Plant science, 2017 - Elsevier
Oils and fats are stored in endosperm during seed development in the form of
triacylglycerols. Three acyltransferases: glycerol-3-phosphate acyltransferase (GPAT) …

Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content

RJ Weselake, S Shah, M Tang, PA Quant… - Journal of …, 2008 - academic.oup.com
Top–down control analysis (TDCA) is a useful tool for quantifying constraints on metabolic
pathways that might be overcome by biotechnological approaches. Previous studies on lipid …

DGAT1, DGAT2 and PDAT Expression in Seeds and Other Tissues of Epoxy and Hydroxy Fatty Acid Accumulating Plants

R Li, K Yu, DF Hildebrand - Lipids, 2010 - Springer
Triacylglycerol (TAG) is the main storage lipid in plants. Acyl-CoA: diacylglycerol
acyltransferase (DGAT1 and DGAT2) and phospholipid: diacylglycerol acyltransferase …

[HTML][HTML] Using lipidomics to reveal details of lipid accumulation in developing seeds from oilseed rape (Brassica napus L.)

HK Woodfield, A Cazenave-Gassiot, RP Haslam… - … et Biophysica Acta (BBA …, 2018 - Elsevier
With dwindling available agricultural land, concurrent with increased demand for oil, there is
much current interest in raising oil crop productivity. We have been addressing this issue by …