Cryopreservation affects the quality of in vitro produced bovine embryos at the molecular level

H Stinshoff, S Wilkening, A Hanstedt, K Brüning… - Theriogenology, 2011 - Elsevier
There are still immense differences in the quality of in vitro produced embryos compared to
their in vivo generated counterparts. These differences include a higher sensitivity of in vitro …

Developmental kinetics of in vitro–produced bovine embryos: an aid for making decisions

S Carrocera, JN Caamaño, B Trigal, D Martín, C Díez - Theriogenology, 2016 - Elsevier
Embryo developmental kinetics and embryo survival after cryopreservation have been
correlated with embryo quality and viability. The main objectives of this work were to analyze …

Basic and applied features in the cryopreservation progress of bovine embryos

RS Valente, TV Marsico, MJ Sudano - Animal Reproduction Science, 2022 - Elsevier
The expansion of the use of in vitro production techniques has revolutionized the bovine
embryo market. In the last decade, we have seen the number of in vitro produced (IVP) …

Effects of different cryopreservation methods on post-thaw culture conditions of in vitro produced bovine embryos

AC Nicacio, R Simões, FF de Paula-Lopes… - Zygote, 2012 - cambridge.org
The aim of this work was to evaluate the effect of cryopreservation protocols on subsequent
development of in vitro produced bovine embryos under different culture conditions …

A comparative analysis of the efficacy of three cryopreservation protocols on the survival of in vitro-derived cattle embryos at pronuclear and blastocyst stages

S Walton, S Catt, AW Taylor-Robinson - Cryobiology, 2017 - Elsevier
The effectiveness of three cryopreservation protocols (slow freezing, short equilibration
vitrification and long equilibration vitrification) on in vitro-derived cattle embryos at expanded …

Cryopreservation of in vitro-produced embryos: challenges for commercial implementation

BV Sanches, AF Zangirolamo… - Animal …, 2018 - animal-reproduction.org
In the last several years, the high demand for embryo production has resulted in the need to
study new methods to make the cryopreservation of bovine embryos produced in vitro more …

Comparison of two-step vitrification versus controlled freezing on survival of in vitro produced cattle embryos

AR Mahmoudzadeh, A Van Soom, MT Ysebaert… - Theriogenology, 1994 - Elsevier
Grade 1 in vitro produced bovine day 7 embryos at the compact morula-early blastocyst,
blastocyst and expanded blastocyst stage were selected for cryopreservation. From 7 …

Survival of vitrified in vitro–produced bovine embryos after a one-step warming in-straw cryoprotectant dilution procedure

JN Caamaño, E Gómez, B Trigal, M Muñoz… - Theriogenology, 2015 - Elsevier
Vitrification is an alternative to slow-rate freezing for cryopreserving bovine embryos.
However, this technology requires simplification if it is to be used under field conditions. The …

Analysis of cryoprotectant, cooling rate and in situ dilution using conventional freezing or vitrification for cryopreserving sheep embryos

MC Schiewe, WF Rall, LD Stuart, DE Wildt - Theriogenology, 1991 - Elsevier
Two studies were conducted to evaluate the influence of cryoprotectant, cooling rate,
container and cryopreservation procedure on the post-thaw viability of sheep embryos. In …

Cryopreservation method affects DNA fragmentation in trophectoderm and the speed of re-expansion in bovine blastocysts

Y Inaba, S Miyashita, T Somfai, M Geshi, S Matoba… - Cryobiology, 2016 - Elsevier
This study investigated re-expansion dynamics during culture of bovine blastocysts
cryopreserved either by slow-freezing or vitrification. Also, the extent and localization of …