Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions
Base editing induces single-nucleotide changes in the DNA of living cells using a fusion
protein containing a catalytically defective Streptococcus pyogenes Cas9, a cytidine …
protein containing a catalytically defective Streptococcus pyogenes Cas9, a cytidine …
CRISPR technologies for genome, epigenome and transcriptome editing
Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …
understanding of CRISPR biology and its application to genome, epigenome and …
Genome-wide target specificity of CRISPR RNA-guided adenine base editors
Adenine base editors enable efficient targeted adenine-to-guanine single nucleotide
conversions to induce or correct point mutations in human cells, animals, and plants,,–. Here …
conversions to induce or correct point mutations in human cells, animals, and plants,,–. Here …
Cytosine base editors with minimized unguided DNA and RNA off-target events and high on-target activity
Y Yu, TC Leete, DA Born, L Young, LA Barrera… - Nature …, 2020 - nature.com
Cytosine base editors (CBEs) enable efficient, programmable reversion of T• A to C• G point
mutations in the human genome. Recently, cytosine base editors with rAPOBEC1 were …
mutations in the human genome. Recently, cytosine base editors with rAPOBEC1 were …
Web-based design and analysis tools for CRISPR base editing
Background As a result of its simplicity and high efficiency, the CRISPR-Cas system has
been widely used as a genome editing tool. Recently, CRISPR base editors, which consist …
been widely used as a genome editing tool. Recently, CRISPR base editors, which consist …
DNA base editing in nuclear and organellar genomes
Genome editing continues to revolutionize biological research. Due to its simplicity and
flexibility, CRISPR/Cas-based editing has become the preferred technology in most systems …
flexibility, CRISPR/Cas-based editing has become the preferred technology in most systems …
Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
K Zheng, Y Wang, N Li, FF Jiang, CX Wu, F Liu… - Communications …, 2018 - nature.com
The ability to precisely edit individual bases of bacterial genomes would accelerate the
investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase …
investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase …
Base editing landscape extends to perform transversion mutation
Base editors have drawn considerable academic and industrial attention in recent years
because of their ability to alter single DNA bases with precision. However, the existing …
because of their ability to alter single DNA bases with precision. However, the existing …
Cytosine base editor 4 but not adenine base editor generates off-target mutations in mouse embryos
Deaminase base editing has emerged as a tool to install or correct point mutations in the
genomes of living cells in a wide range of organisms. However, the genome-wide off-target …
genomes of living cells in a wide range of organisms. However, the genome-wide off-target …
Programmable C: G to G: C genome editing with CRISPR-Cas9-directed base excision repair proteins
L Chen, JE Park, P Paa, PD Rajakumar… - Nature …, 2021 - nature.com
Many genetic diseases are caused by single-nucleotide polymorphisms. Base editors can
correct these mutations at single-nucleotide resolution, but until recently, only allowed for …
correct these mutations at single-nucleotide resolution, but until recently, only allowed for …