Analysis and minimization of cellular RNA editing by DNA adenine base editors
Adenine base editors (ABEs) enable precise and efficient conversion of target A• T base
pairs to G• C base pairs in genomic DNA with a minimum of by-products. While ABEs have …
pairs to G• C base pairs in genomic DNA with a minimum of by-products. While ABEs have …
The next generation of CRISPR–Cas technologies and applications
A Pickar-Oliver, CA Gersbach - Nature reviews Molecular cell biology, 2019 - nature.com
The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …
[PDF][PDF] Target-specific precision of CRISPR-mediated genome editing
AM Chakrabarti, T Henser-Brownhill, J Monserrat… - Molecular cell, 2019 - cell.com
The CRISPR-Cas9 system has successfully been adapted to edit the genome of various
organisms. However, our ability to predict the editing outcome at specific sites is limited …
organisms. However, our ability to predict the editing outcome at specific sites is limited …
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
Y Fu, JA Foden, C Khayter, ML Maeder, D Reyon… - Nature …, 2013 - nature.com
Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases
(RGNs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we …
(RGNs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we …
Dual base editor catalyzes both cytosine and adenine base conversions in human cells
X Zhang, B Zhu, L Chen, L Xie, W Yu, Y Wang… - Nature …, 2020 - nature.com
Although base editors are useful tools for precise genome editing, current base editors can
only convert either adenines or cytosines. We developed a dual adenine and cytosine base …
only convert either adenines or cytosines. We developed a dual adenine and cytosine base …
Synthetic CRISPR RNA-Cas9–guided genome editing in human cells
M Rahdar, MA McMahon, TP Prakash… - Proceedings of the …, 2015 - National Acad Sciences
Genome editing with the c lustered, r egularly i nterspaced, s hort p alindromic r epeats
(CRISPR)-Cas9 nuclease system is a powerful technology for manipulating genomes …
(CRISPR)-Cas9 nuclease system is a powerful technology for manipulating genomes …
Glycosylase-based base editors for efficient T-to-G and C-to-G editing in mammalian cells
L Ye, D Zhao, J Li, Y Wang, B Li, Y Yang, X Hou… - Nature …, 2024 - nature.com
Base editors show promise for treating human genetic diseases, but most current systems
use deaminases, which cause off-target effects and are limited in editing type. In this study …
use deaminases, which cause off-target effects and are limited in editing type. In this study …
[HTML][HTML] HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing
N Liu, L Zhou, G Lin, Y Hu, Y Jiao, Y Wang, J Liu… - … Therapy-Nucleic Acids, 2022 - cell.com
Recent advances in CRISPR-Cas9 techniques, especially the discovery of base and prime
editing, have significantly improved our ability to make precise changes in the genome. We …
editing, have significantly improved our ability to make precise changes in the genome. We …
Programmed genome editing by a miniature CRISPR-Cas12f nuclease
Z Wu, Y Zhang, H Yu, D Pan, Y Wang, Y Wang… - Nature chemical …, 2021 - nature.com
The RNA-guided CRISPR-associated (Cas) nucleases are versatile tools for genome editing
in various organisms. The large sizes of the commonly used Cas9 and Cas12a nucleases …
in various organisms. The large sizes of the commonly used Cas9 and Cas12a nucleases …
DNA capture by a CRISPR-Cas9–guided adenine base editor
CRISPR-Cas–guided base editors convert A• T to G• C, or C• G to T• A, in cellular DNA for
precision genome editing. To understand the molecular basis for DNA adenosine …
precision genome editing. To understand the molecular basis for DNA adenosine …