[HTML][HTML] Improving the genome editing efficiency of CRISPR/Cas9 in Arabidopsis and Medicago truncatula

TW Wolabu, JJ Park, M Chen, L Cong, Y Ge, Q Jiang… - Planta, 2020 - Springer
Main conclusion An improved CRISPR/Cas9 system with the Arabidopsis UBQ10 promoter-
driven Cas9 exhibits consistently high mutation efficiency in Arabidopsis and M. truncatula …

Efficient CRISPR/Cas9-based gene knockout in watermelon

S Tian, L Jiang, Q Gao, J Zhang, M Zong, H Zhang… - Plant Cell Reports, 2017 - Springer
Key message CRISPR/Cas9 system can precisely edit genomic sequence and effectively
create knockout mutations in T0 generation watermelon plants. Abstract Genome editing …

[HTML][HTML] Efficient genome editing using CRISPR/Cas9 technology in chicory

G Bernard, D Gagneul, HAD Santos, A Etienne… - International Journal of …, 2019 - mdpi.com
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR
associated with protein CAS9) is a genome-editing tool that has been extensively used in …

[HTML][HTML] A CRISPR/Cas9 toolkit for multiplex genome editing in plants

HL Xing, L Dong, ZP Wang, HY Zhang, CY Han, B Liu… - BMC plant biology, 2014 - Springer
Background To accelerate the application of the CRISPR/Cas9 (clustered regularly
interspaced short palindromic repeats/CRISPR-associated protein 9) system to a variety of …

[HTML][HTML] A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in Arabidopsis

Z Feng, Z Zhang, K Hua, X Gao, Y Mao… - International journal of …, 2018 - mdpi.com
The CRISPR/Cas9 system has been widely used for targeted genome editing in numerous
plant species. In Arabidopsis, constitutive promoters usually result in a low efficiency of …

[HTML][HTML] Challenges facing CRISPR/Cas9-based genome editing in plants

S Son, SR Park - Frontiers in plant science, 2022 - frontiersin.org
The development of plant varieties with desired traits is imperative to ensure future food
security. The revolution of genome editing technologies based on the clustered regularly …

[HTML][HTML] Modern trends in plant genome editing: an inclusive review of the CRISPR/Cas9 toolbox

A Razzaq, F Saleem, M Kanwal, G Mustafa… - International journal of …, 2019 - mdpi.com
Increasing agricultural productivity via modern breeding strategies is of prime interest to
attain global food security. An array of biotic and abiotic stressors affect productivity as well …

[HTML][HTML] Expanding the range of CRISPR/Cas9 genome editing in rice

X Hu, C Wang, Y Fu, Q Liu, X Jiao, K Wang - Molecular plant, 2016 - cell.com
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …

[HTML][HTML] An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana

R Wu, M Lucke, Y Jang, W Zhu, E Symeonidi, C Wang… - Plant methods, 2018 - Springer
Background Our knowledge of natural genetic variation is increasing at an extremely rapid
pace, affording an opportunity to come to a much richer understanding of how effects of …

[HTML][HTML] Efficient Genome Editing in Populus Using CRISPR/Cas12a

Y An, Y Geng, J Yao, C Fu, M Lu, C Wang… - Frontiers in Plant …, 2020 - frontiersin.org
The ability to create targeted mutations using clustered regularly inter-spaced short
palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 in support of forest tree …