The rapid preparation of multimilligram quantities of five heparin-derived oligosaccharides
(1–5) is described. These oligosaccharides are the final products obtained from the action of …

RJ Linhardt,**'* JE Turnbull, § HM Wang,* D. Loganathan,* and JT Gallagher11 Division of
Medicinal and Natural Products Chemistry, College of Pharmacy, University of Iowa, Iowa …

Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large
scale using beparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2. 2.8) …

Eleven tetrasaccharides were isolated from the repeating disaccharide region of porcine
intestinal heparin after strong digestion with Flavobacterium heparinase. Their structures …

Heparinase and heparitinase were separated from an extract of Flavobacterium heparinum,
induced with heparin by using column chromatography on hydroxylapatite. As the …

Revised Manuscript Received May 4, 1993 abstract: An understanding of the substrate
specificity study of the heparin lyases (heparinase and heparitinases) is crucial for …

Publisher Summary This chapter discusses the structural aspects and structure–activity
relationships of heparin/heparan sulfate (HS). The molecular mechanism behind the …

Heparin lyase I has been purified from Flavobacterium heparinum and has been partially
characterized (Yang, VC, Linhardt, RJ, Berstein, H., Cooney, CL, and Langer, R.(1985) J …

We prepared a series of oligosaccharides from porcine intestinal heparin after extensive
digestion with a mixture of Flavobacterium heparinase as well as heparitinases I and II …

Materials and Methods Analysis. The following were analyzed by the procedures indicated:
uronic acid colorimetrically by the carbazole pro-cedure (Dische, 1947), reducing sugar by …