Background Autotransporters represent a widespread family of secreted proteins in Gram-
negative bacteria. Their seemingly easy secretion mechanism and modular structure make …

Highlights•Autotransporters have predominantly been used for surface display on E.
coli.•For industrial applications, host bacteria with specific features are needed.•Here we …

Autotransporters are a widespread family of proteins, generally known as virulence factors
produced by Gram-negative bacteria. In this study, the esterase A (EstA) autotransporter of …

The functional expression of proteins on the surface of bacteria has proven important for
numerous biotechnological applications. In this report, we investigated the N-terminal fusion …

Cell surface display of proteins can be used for several biotechnological applications such
as the screening of protein libraries, whole cell biocatalysis and live vaccine development …

Pseudomonas putida can be used as a host for the autotransporter‐mediated surface
display of enzymes (autodisplay), resulting in whole‐cell biocatalysts with recombinant …

Expressing proteins of interest as fusions to proteins of the bacterial envelope is a powerful
technique with many biotechnological and medical applications. Autotransporters have …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

Autotransporters constitute a large family of natural proteins that are essential for delivering
many types of proteins and peptides across the outer membrane in Gram-negative bacteria …

The classical monomeric autotransporters are ubiquitously used by Gram-negative bacteria
to export virulence and colonization factors to their cell surface or into their surroundings …