Optimization of RNA extraction protocols using different maize tissues.

BN Porto, PC Magalhães, NA Campos, JD Alves… - 2010 - cabidigitallibrary.org
Five RNA extraction methods were tested (CTAB micro extraction, ConcertTM-Invitrogen;
ConcertTM adapted; TRI Reagent®-Sigma; TRI Reagent® adapted) using two different …

A Simple and efficient protocol for isolation of RNA from different tissues of chickpea and pea

JS Patel, RS Upadhyay, HB Singh… - International Journal …, 2016 - indianjournals.com
A simple and efficient protocol is developed for isolation of high quality RNA from roots and
leaves of chickpea and pea. The procedure is based on use of SDS, sodium acetate and …

The MAGi RNA extraction method: a highly efficient and simple procedure for fresh and dry plant tissues

AG Ince, M Karaca - Journal of the Science of Food and …, 2009 - Wiley Online Library
BACKGROUND: Samples from different plant species, different organs or tissues at different
times of the year, usually show great differences in their cell compositions, pH, and the …

RNA extraction from cereal vegetative tissue

JA Pattemore - Cereal Genomics: Methods and Protocols, 2014 - Springer
Ribonucleic acid (RNA) extraction is the necessary first step in many protocols, primarily to
investigate genes and gene expression. RNA comes in a variety of forms: total RNA …

Optimized mRNA Isolation and Assessment Methodology with Two‐Step Real Time PCR

C Psallida, D Argyropoulos - Preparative Biochemistry and …, 2005 - Taylor & Francis
Messenger ribonucleic acid (mRNA) isolation from tomato seeds was performed with the
PolyATtract® System 1000 and was evaluated in two‐step Real Time PCR using the …

RNA extraction

H Shi, R Bressan - Arabidopsis protocols, 2006 - Springer
RNA extraction is a routine technique in a molecular biology lab. High quality of RNA
extracted from plants is a prerequisite to succeeding in subsequent experiments such as …

[HTML][HTML] Rapid and reliable method of high-quality RNA extraction from diverse plants

SK Sah, G Kaur, A Kaur - American Journal of Plant Sciences, 2014 - scirp.org
The isolation of high quality RNA is a crucial technique in plant molecular biology. The
quality of RNA determines the reliability of downstream process like real time PCR. In this …

A comparison of commercially-available automated and manual extraction kits for the isolation of total RNA from small tissue samples

MK Sellin Jeffries, AJ Kiss, AW Smith, JT Oris - BMC biotechnology, 2014 - Springer
Background This study compared the performance of five commercially available kits in
extracting total RNA from small eukaryotic tissue samples (< 15 mg). Total RNA was isolated …

Modified cetyltrimethylammonium bromide method improves robustness and versatility: the benchmark for plant RNA extraction

EJ White, M Venter, NF Hiten, JT Burger - 2008 - Wiley Online Library
A wide range of plant RNA extraction methods are available; however, many of these are
limited in their application for a diverse range of plant species. With special emphasis on …

A Simple and Efficient Protocol of RNA Extraction from Apple Leaves (Malus x domestica); A Silica Column-Based Method

S Zulaeha, A Safarrida, A Suyono… - … of Agriculture System, 2023 - pasca.unhas.ac.id
Extracting total RNA from plant tissue using the conventional isolation method is time-
consuming, Â requires expensive reagents and produces low-quality RNA. Over time …