The combination of Gilbert/Maxam chemical sequencing and the dideoxynucleotide chain termination approach facilitates the construction of species specific PCR …

K Voigt, J Wöstemeyer - Microbiological research, 1995 - Elsevier
The randomly amplified polymorphic DNA technique (RAPD) is a modification of PCR that
uses short, arbitrarily generated single primers to amplify genomic DNA. Amplified DNA …

[PDF][PDF] Factors affecting reliability and reproducibility of amplification-based DNA fingerprinting of representative bacterial pathogens

KD Tyler, G Wang, SD Tyler… - Journal of Clinical …, 1997 - Am Soc Microbiol
Arbitrary amplification of polymorphic DNA sequences has increasingly been reported as a
method for the genetic characterization of microorganisms, and there are many variations of …

RAPD- and ERIC-Based Typing of Clinical and Environmental Pseudomonas aeruginosa Isolates

IG Auda, IMS Al-Kadmy, SM Kareem… - Journal of AOAC …, 2017 - academic.oup.com
Pseudomonas aeruginosa is a major cause of nosocomial infection in children and adults,
resulting in significant morbidity and mortality due to its ability to acquire drug resistance …

Rapid identification of biothreat and other clinically relevant bacterial species by use of universal PCR coupled with high-resolution melting analysis

S Yang, P Ramachandran, R Rothman… - Journal of clinical …, 2009 - Am Soc Microbiol
ABSTRACT A rapid assay for eubacterial species identification is described using high-
resolution melt analysis to characterize PCR products. Unique melt profiles generated from …

Classification, identification and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments

J Jonasson, M Olofsson, HJ Monstein - Apmis, 2002 - Wiley Online Library
The rapid identification of the etiological agent of microbial infections can bring about both
clinical and financial benefits. Thus, fast and generally applicable classification methods are …

[HTML][HTML] Speciation of common Gram-negative pathogens using a highly multiplexed high resolution melt curve assay

T Edwards, S Sasaki, C Williams, G Hobbs… - Scientific Reports, 2018 - nature.com
The identification of the bacterial species responsible for an infection remains an important
step for the selection of antimicrobial therapy. Gram-negative bacteria are an important …

[HTML][HTML] Inaccurate multilocus sequence typing of Acinetobacter baumannii

S Castillo-Ramírez, L Graña-Miraglia - Emerging infectious …, 2019 - ncbi.nlm.nih.gov
Multilocus sequence typing has been useful for genotyping pathogens in surveillance and
epidemiologic studies. However, it cannot reflect the true relationships of isolates for species …

Establishment and evaluation of a core genome multilocus sequence typing scheme for whole-genome sequence-based typing of Pseudomonas aeruginosa

H Tönnies, K Prior, D Harmsen… - Journal of clinical …, 2021 - Am Soc Microbiol
The environmental bacterium Pseudomonas aeruginosa, particularly multidrug-resistant
clones, is often associated with nosocomial infections and outbreaks. Today, core genome …

Evaluation of a PCR melting profile technique for bacterial strain differentiation

B Krawczyk, A Samet, J Leibner… - Journal of clinical …, 2006 - Am Soc Microbiol
In search of an effective DNA typing technique for hospital epidemiology use, the
performance and convenience of a PCR melting profile (PCR MP) technique based on using …

Phylogenetic Classification of Pseudomonas putida Strains by MALDI-MS Using Ribosomal Subunit Proteins as Biomarkers

K Teramoto, H Sato, L Sun, M Torimura, H Tao… - Analytical …, 2007 - ACS Publications
A new method for phylogenetic classification of bacterial strains using matrix-assisted laser
desorption/ionization-mass spectrometry (MALDI-MS) is proposed. This method was …