[HTML][HTML] Genome editing in bacteria: CRISPR-Cas and beyond
RD Arroyo-Olarte, R Bravo Rodriguez, E Morales-Ríos - Microorganisms, 2021 - mdpi.com
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
Barriers to genome editing with CRISPR in bacteria
Genome editing is essential for probing genotype–phenotype relationships and for
enhancing chemical production and phenotypic robustness in industrial bacteria. Currently …
enhancing chemical production and phenotypic robustness in industrial bacteria. Currently …
[HTML][HTML] Harnessing CRISPR–Cas systems for bacterial genome editing
K Selle, R Barrangou - Trends in microbiology, 2015 - cell.com
Manipulation of genomic sequences facilitates the identification and characterization of key
genetic determinants in the investigation of biological processes. Genome editing via …
genetic determinants in the investigation of biological processes. Genome editing via …
Multiplex genome editing of microorganisms using CRISPR-Cas
B Adiego-Perez, P Randazzo, JM Daran… - FEMS microbiology …, 2019 - academic.oup.com
Microbial production of chemical compounds often requires highly engineered microbial cell
factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful …
factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful …
Bacterial Genome Editing with CRISPR-Cas9: Deletion, Integration, Single Nucleotide Modification, and Desirable “Clean” Mutant Selection in Clostridium beijerinckii …
CRISPR-Cas9 has been demonstrated as a transformative genome engineering tool for
many eukaryotic organisms; however, its utilization in bacteria remains limited and …
many eukaryotic organisms; however, its utilization in bacteria remains limited and …
[HTML][HTML] Endogenous CRISPR-Cas system-based genome editing and antimicrobials: review and prospects
Y Li, N Peng - Frontiers in microbiology, 2019 - frontiersin.org
CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR …
[HTML][HTML] Evolution of CRISPR/cas systems for precise genome editing
M Hryhorowicz, D Lipiński, J Zeyland - International Journal of Molecular …, 2023 - mdpi.com
The bacteria-derived CRISPR/Cas (an acronym for regularly interspaced short palindromic
repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and …
repeats/CRISPR-associated protein) system is currently the most widely used, versatile, and …
A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome editing in Escherichia coli
Q Li, B Sun, J Chen, Y Zhang… - Acta Biochimica et …, 2021 - academic.oup.com
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated
nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established …
nuclease 9 (Cas9)-based genome editing tool pCas/pTargetF system that we established …
Cas9-based tools for targeted genome editing and transcriptional control
Development of tools for targeted genome editing and regulation of gene expression has
significantly expanded our ability to elucidate the mechanisms of interesting biological …
significantly expanded our ability to elucidate the mechanisms of interesting biological …
[HTML][HTML] Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
Application of CRISPR-Cas9 systems has revolutionized genome editing across all domains
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …
of life. Here we report implementation of the heterologous Type II CRISPR-Cas9 system in …