Rapid Evolution of Diminished Transformability in Acinetobacter baylyi

JM Bacher, D Metzgar… - Journal of bacteriology, 2006 - Am Soc Microbiol
The reason for genetic exchange remains a crucial question in evolutionary biology.
Acinetobacter baylyi strain ADP1 is a highly competent and recombinogenic bacterium. We …

Growth phase-specific evolutionary benefits of natural transformation in Acinetobacter baylyi

ALG Utnes, V Sørum, N Hülter, R Primicerio… - The ISME …, 2015 - academic.oup.com
Natural transformation in bacteria facilitates the uptake and genomic integration of
exogenous DNA. This allows horizontal exchange of adaptive traits not easily achieved by …

Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution

BA Renda, A Dasgupta, D Leon… - Journal of bacteriology, 2015 - Am Soc Microbiol
Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic
biology because it is naturally transformable. To examine the genetic reliability of this …

Natural transformation as a tool in Acinetobacter baylyi: streamlined engineering and mutational analysis

SR Bedore, EL Neidle, I Pardo, J Luo, AC Baugh… - Methods in …, 2023 - Elsevier
Natural transformation and homologous recombination in a soil bacterium, Acinetobacter
baylyi ADP1, occur with exceptionally high efficiency. These genetic features can be …

Costs and benefits of natural transformation in Acinetobacter baylyi

N Hülter, V Sørum, K Borch-Pedersen, MM Liljegren… - BMC microbiology, 2017 - Springer
Background Natural transformation enables acquisition of adaptive traits and drives genome
evolution in prokaryotes. Yet, the selective forces responsible for the evolution and …

Expression vectors for Acinetobacter baylyi ADP1

CD Murin, K Segal, A Bryksin… - Applied and …, 2012 - Am Soc Microbiol
Acinetobacter baylyi ADP1 is naturally competent and proficient at homologous
recombination, so it can be transformed without restriction digests or ligation reactions …

OPPORTUNITIES FOR GENETIC INVESTIGATION AFFORDED BY ACINETOBACTER BAYLYI, A NUTRITIONALLY VERSATILE BACTERIAL SPECIES THAT IS …

DM Young, D Parke, LN Ornston - Annu. Rev. Microbiol., 2005 - annualreviews.org
The genetic and physiological properties of Acinetobacter baylyi strain ADP1 make it an
inviting subject for investigation of the properties underlying its nutritional versatility. The …

Testing for the fitness benefits of natural transformation during community-embedded evolution

M Winter, K Harms, PJ Johnsen, A Buckling… - …, 2023 - microbiologyresearch.org
Natural transformation is a process where bacteria actively take up DNA from the
environment and recombine it into their genome or reconvert it into extra-chromosomal …

Reduced mutation rate and increased transformability of transposon-free Acinetobacter baylyi ADP1-ISx

GA Suárez, BA Renda, A Dasgupta… - Applied and …, 2017 - Am Soc Microbiol
The genomes of most bacteria contain mobile DNA elements that can contribute to
undesirable genetic instability in engineered cells. In particular, transposable insertion …

Sexual Isolation in Acinetobacter baylyi Is Locus-Specific and Varies 10,000-Fold Over the Genome

JL Ray, K Harms, OG Wikmark, I Starikova… - Genetics, 2009 - academic.oup.com
Naturally transformable bacteria acquire chromosomal DNA from related species at lower
frequencies than from cognate DNA sources. To determine how genome location affects …