Bone-forming cells fabricate a highly ordered collagen matrix (osteoid) which subsequently
mineralizes. A variety of cell culture systems exist for osteogenic cells, yet none of these is …

Bone can be described as a highly ordered composite of type I collagen integrated with an
inorganic mineral phase. In vitro models of bone mineralization using collagenous …

An in-vitro method of mineralizing an aligned, self-assembled collagenous matrix is
presented. Reconstituted collagen fibers were mineralized by exposure to saturated …

When grown in medium containing ascorbic acid and β‐glycerol phosphate, mouse MC3T3‐
E1 cells express an osteoblast phenotype and produce a highly mineralized extracellular …

Osteoblasts isolated mechanically from newborn mouse calvaria produced a calcified matrix
when cultured in the presence of 10 mM β-glycerophosphate or 3 mM inorganic phosphate …

The scaffold upon which bone is synthesized is predominantly type I collagen. Although
minor collagens do exist in bone, such as types III, V and VI [38, 51, 67], their role in the …

Collagen fibers self-assembled from solutions of molecular type I collagen were mineralized
at pH 9.5, by exposure to super-saturated solutions of calcium and phosphate for a one …

A collagenous matrix with ultrastructural properties analogous to tendon serves as a
substrate for the nucleation and growth of bone-like hydroxyapatite crystals in an acellular in …

Osteoblasts from calvaria of 18‐day‐old fetal Sprague‐Dawley rats were isolated using a
dissecting procedure followed by collagenase digestion. Freshly isolated or previously …

A newly defined chick calvariae osteoblast culture system that undergoes a temporal
sequence of differentiation of the osteoblast phenotype with subsequent mineralization …