Background It is widely believed that laboratory strains of Escherichia coli, including those
used for industrial production of proteins, do not secrete proteins to the extracellular milieu …

Escherichia coli is still a very popular host for the production of recombinant proteins at an
analytical or industrial scale. Secretion of the proteins into the culture medium or display at …

The secretory production of recombinant proteins by the Gram-negative bacterium
Escherichia coli has several advantages over intracellular production as inclusion bodies. In …

During the past few years, significant progress has been made towards our understanding of
the molecular mechanisms governing the translocation of proteins through bacterial cell …

Extracellular production of heterologous proteins using the Escherichia coli cell factory offers
several advantages over intracellular production and mammalian culture. Properly folded …

The microorganism Escherichia coli is commonly used for recombinant protein production.
Despite several advantageous characteristics like fast growth and high protein yields, its …

A method for enhancing the secretion of heterologous proteins in Escherichia coli by
optimizing, as opposed to simply maximizing, the translational level of a given protein is …

Recent years have witnessed significant progresses in engineering of recombinant protein
secretion. The relatively simple secretion mechanisms, Type I and Type V (autotransporters) …

Escherichia coli is the most commonly used host for recombinant protein production and
metabolic engineering. Extracellular production of enzymes and proteins is advantageous …

Recombinant protein expression in Escherichia coli represents a cornerstone of the
biotechnology enterprise. While cytoplasmic expression in this host has received the most …