Citrus is a highly valued tree crop worldwide, while, at the same time, citrus production faces
many biotic challenges, including bacterial canker and Huanglongbing (HLB). Breeding for …

Worldwide, citrus is one of the most important fruit crops and is grown in more than 130
countries, predominantly in tropical and subtropical areas. The healthy progress of the citrus …

Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …

Summary Recently, CRISPR‐Cas12a (Cpf1) from Prevotella and Francisella was
engineered to modify plant genomes. In this report, we employed CRISPR‐LbCas12a …

Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the
global citrus industry. Targeted editing of host disease‐susceptibility genes represents an …

Citrus is one of the most important fruit crops in the world. Global commercial orange (Citrus
sinensis) production exceeded 47 million tons (mt) in 2015 (https://apps. fas. usda …

Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …

Genetic modification, including plant breeding, has been widely used to improve crop yield
and quality, as well as to increase disease resistance. Targeted genome engineering is …

Citrus canker caused by Xanthomonas citri subsp. citri is one of the most devastating citrus
diseases worldwide. Generating disease-resistant citrus varieties is considered one of the …

Key message We have developed multiplex genome editing toolkits for citrus that
significantly improve citrus genome editing efficacy. Abstract CRISPR/Cas systems have …