Viable piglets generated from porcine oocytes matured in vitro and fertilized by intracytoplasmic sperm head injection

M Nakai, N Kashiwazaski, A Takizawa… - Biology of …, 2003 - academic.oup.com
Intracytoplasmic sperm injection (ICSI) of a nonmotile cell into the ooplasm for assisted
fertilization is a highly specialized procedure for producing the next generation. The …

Development of in vivo-matured porcine oocytes following intracytoplasmic sperm injection

MJ Martin - Biology of Reproduction, 2000 - academic.oup.com
The objective of this study was to assess the development of porcine ova fertilized by
intracytoplasmic sperm injection (ICSI). Allyl trenbolone (Regumate) was used to …

Birth of a piglet derived from an oocyte fertilized by intracytoplasmic sperm injection (ICSI)

T Kolbe, W Holtz - Animal Reproduction Science, 2000 - Elsevier
This report is of a live piglet derived from an embryo produced by intracytoplasmic sperm
injection and carried to term. In vivo-matured oocytes were injected with in vitro-capacitated …

Production of piglets using intracytoplasmic sperm injection (ICSI) with flowcytometrically sorted boar semen and artificially activated oocytes

S Probst, D Rath - Theriogenology, 2003 - Elsevier
The purpose of the present study was to develop a protocol for the successful production of
piglets employing intracytoplasmic sperm injection (ICSI) with flowcytometrically sexed …

In vitro maturation of porcine oocytes using a defined medium and developmental capacity after intracytoplasmic sperm injection

R Kishida, ES Lee, Y Fukui - Theriogenology, 2004 - Elsevier
To establish a defined in vitro maturation culture system for porcine oocytes, we examined
the effects of adding cysteine (Cys) and epidermal growth factor (EGF) to the maturation …

Development of porcine embryos and offspring after intracytoplasmic sperm injection with liposome transfected or non-transfected sperm into in vitro matured oocytes

L Lai, Q Sun, G Wu, CN Murphy, B Kühholzer, KW Park… - Zygote, 2001 - cambridge.org
The objective of this study was to evaluate in vitro and in vivo development of porcine in vitro
matured (IVM) porcine oocytes fertilised by intracytoplasmic sperm injection (ICSI) and the …

Experiments to improve in vitro fertilization techniques for in vivo-matured porcine oocytes

D Rath - Theriogenology, 1992 - Elsevier
Three experiments were designed for the in vitro fertilization of in vivo matured oocytes
following different sperm treatments: In experiment I, spermatozoa (sperm rich fraction) were …

Failure of male pronucleus formation is the major cause of lack of fertilization and embryo development in pig oocytes subjected to intracytoplasmic sperm injection

JW Lee, XC Tian, X Yang - Biology of Reproduction, 2003 - academic.oup.com
The objectives of this study were 1) to compare the efficiency of intracytoplasmic sperm
injection (ICSI) with and without additional artificial stimulation using frozen-thawed sperm …

Effect of short periods of sperm–oocyte coincubation during in vitro fertilization on embryo development in pigs

MA Gil, M Ruiz, JM Vazquez, J Roca, BN Day… - Theriogenology, 2004 - Elsevier
The present study was conducted to determine if the efficiency of in vitro pig embryo
production could be improved by a reduction in the period of time that oocytes are exposed …

Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y chromosome bearing spermatozoa sorted by high speed …

LR Abeydeera, LA Johnson, GR Welch, WH Wang… - Theriogenology, 1998 - Elsevier
The present study examined the ability to establish pregnancies after transfer of pig embryos
derived from in vitro fertilization (IVF) of in vitro matured (IVM) oocytes by X and Y …