A sufficient amount of easily obtained and well-characterized osteoblastic cells is a useful
tool to study biomaterial/cell interactions essential for bone tissue engineering. Osteoblastic …

Mineralized extracellular matrix formation is representative for the osteoinductive capacity of
biomaterials and is often tested in vitro. Characteristics of in vitro mineralization of primary …

Calvaria from neonatal mouse and rat is ideal resource for osteoblasts but can be easily
contaminated by other cells such as fibroblasts. Here, we established a protocol for isolation …

The aim of our study was to investigate the influence of four bone substitutes on the growth
behavior of a human osteoblast‐like cell line (SaOS‐2) culture: pure alpha tricalcium …

Many biomaterials have been evaluated using cultured cells. In particular, osteoblast-like
cells are often used to evaluate the osteocompatibility, hard-tissue-regeneration …

Bone regeneration after injury or after surgical bone removal due to disease is a serious
medical challenge. A variety of materials are being tested to replace a missing bone or tooth …

The effects of biocompatible ceramics on the growth and adhesion of osteoblast-rich rat
calvarial cell cultures were investigated. Osteoblast-like cells and mouse fibroblast-like L …

This chapter describes the isolation of primary mouse osteoblasts from adult mouse calvaria
and long bones, as well as the process of isolation of bone cells from neonatal mouse …

The process of bone formation, remodelling and healing involves a coordinated action of
various cell types. Advances in understanding the biology of osteoblast cells during these …

Bone marrow stromal cells of femora from young adult rats produce bone-like mineralized
tissue in culture. We evaluated three implant materials (hydroxyapatite (HA), glass-ceramics …