The synthesis of proteins with a fully native sequence is an ongoing challenge in protein
chemistry. Native chemical ligation (NCL) approaches have proven to be generally …

The discovery of native chemical ligation (NCL) by Kent and co-workers in 1994 was a
critical step, enabling researchers to chemically access medium-sized proteins through total …

Chemoselective peptide ligation methods have provided synthetic access to numerous
proteins, including those bearing native post-translational modifications and unnatural …

Of the many approaches proposed to generalize the native chemical ligation approach for
protein synthesis, the simple procedure of global desulfurization of peptide thiols has …

A convergent strategy for glycoprotein synthesis entails the assembly of individual peptidyl
substrates, each bearing a single carbohydrate domain. These subunits would then be …

The development of site‐selective chemistry targeting the canonical amino acids enables
the controlled installation of desired functionalities into native peptides and proteins. Such …

Although native chemical ligation has enabled the synthesis of hundreds of proteins, not all
proteins are accessible through typical ligation conditions. The challenging protein, 125 …

We describe an unprecedented reaction between peptide selenoesters and peptide dimers
bearing N-terminal selenocystine that proceeds in aqueous buffer to afford native amide …

The highly chemoselective reaction between unprotected peptides bearing an N-terminal
Cys residue and a C-terminal thioester enables the total and semi-synthesis of complex …

Cysteine‐based native chemical ligation (NCL) has been a very powerful approach for
convergent synthesis of peptides and proteins. However, owing to the low abundance of …