Fusion competence of phosphatidylserine-containing liposomes quantitatively measured by a fluorescence resonance energy transfer assay

PS Uster, DW Deamer - Archives of Biochemistry and Biophysics, 1981 - Elsevier
A sensitive, quantitative assay has been developed which measures the extent of liposome
fusion by monitoring fluorescence resonance energy transfer between two lipid analogs …

Role of lipid phase separations and membrane hydration in phospholipid vesicle fusion

D Hoekstra - Biochemistry, 1982 - ACS Publications
Dick Hoekstra** abstract: The relationship between lipid phase separation and fusion of
small unilamellar phosphatidylserine-containing vesicles was investigated. The kinetics of …

Studies on the mechanism of membrane fusion. Role of head-group composition in calcium-and magnesium-induced fusion of mixed phospholipid vesicles

N Düzgünes, J Wilschut, R Fraley… - … et Biophysica Acta (BBA …, 1981 - Elsevier
We have investigated the contribution of various phospholipids to membrane fusion induced
by divalent cations. Fusion was followed by means of a new fluorescence assay monitoring …

Monitoring of phospholipid vesicle fusion by fluorescence energy transfer between membrane-bound dye labels

P Vanderwerf, EF Ullman - Biochimica et Biophysica Acta (BBA) …, 1980 - Elsevier
A sensitive method which utilizes fluorescence energy transfer to assay Ca 2+-or Mg 2+-
mediated fusion of phospholipid vesicles is reported. More than 85% quenching results …

Membrane fusion and molecular segregation in phospholipid vesicles

D Papahadjopoulos, G Poste, BE Schaeffer… - Biochimica et biophysica …, 1974 - Elsevier
Fusion between vesicles prepared from individual or mixed phospholipid species was
demonstrated by ultracentrifugation and gel-filtration techniques, electron microscopy and …

Comparison of two liposome fusion assays monitoring the intermixing of aqueous contents and of membrane components

J Rosenberg, N Düzgüneş, Ç Kayalar - Biochimica et Biophysica Acta (BBA …, 1983 - Elsevier
Divalent cation-induced fusion of large unilamellar vesides (approx. 0.1 μm diameter) made
of phosphatidylserine (PS) or phosphatidylglycerol (PG) has been studied. Intermixing of …

Proton-induced fusion of oleic acid-phosphatidylethanolamine liposomes

N Duzgunes, RM Straubinger, PA Baldwin… - Biochemistry, 1985 - ACS Publications
Revised Manuscript Received November 29, 1984 abstract: Liposomes composed of oleic
acid and phosphatidylethanolamine (3: 7 mole ratio) aggregate, become destabilized, and …

Fluorescence assays for membrane fusion

N DÜzgÜneş, J Bentz - Spectroscopic Membrane Probes, 2018 - taylorfrancis.com
This chapter examines assays for membrane fusion: two assays for the intermixing of
aqueous contents of phospholipid vesicles, and one for the mixing of membrane …

Retention of aqueous contents during divalent cation-induced fusion of phospholipid vesicles

J Wilschut, N Düzgünes, K Hong, D Hoekstra… - … et Biophysica Acta (BBA …, 1983 - Elsevier
The relative kinetics of intermixing and release of liposome aqueous contents during Ca 2+-
induced membrane fusion has been investigated. Fusion was monitored by the Tb …

Use of resonance energy transfer to monitor membrane fusion

DK Struck, D Hoekstra, RE Pagano - Biochemistry, 1981 - ACS Publications
Douglas K. Struck,** Dick Hoekstra, and Richard E. Pagano* abstract: An assay for vesicle-
vesicle fusion involving resonance energy transfer between 7V-(7-nitro-2, l, 3-benz …