GAL4 activates gene expression in mammalian cells
H Kakidani, M Ptashne - Cell, 1988 - cell.com
Results GAL4, a protein that activates transcription in yeast, is shown to activate the mouse
mammary tumor virus promoter in mammalian cells. Activation depends upon a GAL4 …
mammary tumor virus promoter in mammalian cells. Activation depends upon a GAL4 …
Mutants of GAL4 protein altered in an activation function
G Gill, M Ptashne - Cell, 1987 - cell.com
Activating region I of GAL4 has been defined as a region 48 amino acids long which, when
attached to GAL4's DNA-binding domain, activates transcription in yeast. Here we describe …
attached to GAL4's DNA-binding domain, activates transcription in yeast. Here we describe …
A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor
R Brent, M Ptashne - Cell, 1985 - cell.com
We describe a new protein that binds to DNA and activates gene transcription in yeast. This
protein, LexA-GAL4, is a hybrid of LexA, an Escherichia coii repressor protein, and GAL4, a …
protein, LexA-GAL4, is a hybrid of LexA, an Escherichia coii repressor protein, and GAL4, a …
GAL4 is phosphorylated as a consequence of transcriptional activation.
I Sadowski, D Niedbala, K Wood… - Proceedings of the …, 1991 - National Acad Sciences
GAL4 protein isolated from yeast in which it is active is phosphorylated predominantly on
two different serine residues. One of these was identified as Ser-837; substitution of this …
two different serine residues. One of these was identified as Ser-837; substitution of this …
Cooperative DNA binding of the yeast transcriptional activator GAL4.
E Giniger, M Ptashne - … of the National Academy of Sciences, 1988 - National Acad Sciences
The yeast transcriptional regulatory protein GAL4 binds to four sites in the GAL upstream
activating sequence and stimulates transcription of the adjacent GAL1 and GAL10 genes …
activating sequence and stimulates transcription of the adjacent GAL1 and GAL10 genes …
Negative effect of the transcriptional activator GAL4
G Gill, M Ptashne - Nature, 1988 - nature.com
The yeast transcriptional activator GAL4 binds specific sites on DNA to activate transcription
of adjacent genes1–5. The distinct activating regions of GAL4 are rich in acidic residues and …
of adjacent genes1–5. The distinct activating regions of GAL4 are rich in acidic residues and …
A vector for expressing GAL4 (1-147) fusions in mammalian cells.
I Sadowski, M Ptashne - Nucleic Acids Research, 1989 - ncbi.nlm.nih.gov
Transcriptional activator proteins bind to specific enhancer elements in eukaryotic
promoters, and stimulate transcription of nearby genes (1). Separable functional elements of …
promoters, and stimulate transcription of nearby genes (1). Separable functional elements of …
Yeast activators stimulate plant gene expression
J Ma, E Przibilla, J Hu, L Bogorad, M Ptashne - Nature, 1988 - nature.com
GAL4 is a transcriptional activator found in yeast1, 2. Two distinct functions of the protein are
required for its activity3–5: one directs sequen1ce-specific DNA binding, and another …
required for its activity3–5: one directs sequen1ce-specific DNA binding, and another …
Deletion analysis of GAL4 defines two transcriptional activating segments
J Ma, M Ptashne - Cell, 1987 - cell.com
We describe the activities of a wlde array of deletion mutants of GAL4, a yeast transcriptional
activator. We identify two short regions of GAL4, each of which activates transcription when …
activator. We identify two short regions of GAL4, each of which activates transcription when …
The hormone-binding domains of the estrogen and glucocorticoid receptors contain an inducible transcription activation function
NJG Webster, S Green, JR Jin, P Chambon - Cell, 1988 - cell.com
We have constructed novel chimeric receptors, GALER and GAL-GR, consisting of the DNA-
binding domain of the yeast transcription factor GAL4 joined to the C-terminal region …
binding domain of the yeast transcription factor GAL4 joined to the C-terminal region …