Development and validation of a rapid, single-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) system potentially to be used for reliable …

M Jiang, W Pan, A Arasthfer, W Fang, L Ling… - Frontiers in cellular …, 2020 - frontiersin.org
Objectives: Development and validation of a single-step and accurate reverse transcriptase
loop-mediated isothermal amplification technique (RT-LAMP) for rapid identification of …

Optimization and clinical validation of colorimetric reverse transcription loop-mediated isothermal amplification, a fast, highly sensitive and specific COVID-19 …

PA Alves, EG de Oliveira, APM Franco-Luiz… - Frontiers in …, 2021 - frontiersin.org
The coronavirus disease 2019 (COVID-19) pandemic unfolded due to the widespread
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission reinforced the …

Development of a reverse transcription-loop-mediated isothermal amplification as a rapid early-detection method for novel SARS-CoV-2

YH Baek, J Um, KJC Antigua, JH Park… - Emerging microbes & …, 2020 - Taylor & Francis
The previous outbreaks of SARS-CoV and MERS-CoV have led researchers to study the
role of diagnostics in impediment of further spread and transmission. With the recent …

Rapid detection of novel coronavirus (COVID-19) by reverse transcription-loop-mediated isothermal amplification

LE Lamb, SN Bartolone, E Ward, MB Chancellor - MedRxiv, 2020 - medrxiv.org
Novel Corona virus (COVID-19 or 2019-nCoV) is an emerging global health concern that
requires a rapid diagnostic test. Quantitative reverse transcription PCR (qRT-PCR) is …

Clinical COVID-19 diagnostic methods: Comparison of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and quantitative RT-PCR (qRT-PCR)

H Kitajima, Y Tamura, H Yoshida, H Kinoshita… - Journal of Clinical …, 2021 - Elsevier
Background The coronavirus disease 2019 (COVID-19) pandemic is a major public health
concern. Accurate and rapid diagnosis of COVID-19 is critical for disease control. Reverse …

Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus …

K Nawattanapaiboon, E Pasomsub, P Prombun… - Analyst, 2021 - pubs.rsc.org
COVID-19, caused by the infection of SARS-CoV-2, has emerged as a rapidly spreading
infection. The disease has now reached the level of a global pandemic and as a result a …

A highly effective reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 infection

VL Fowler, B Armson, JL Gonzales, EL Wise… - Journal of infection, 2021 - Elsevier
The COVID-19 pandemic has illustrated the importance of simple, rapid and accurate
diagnostic testing. This study describes the validation of a new rapid SARS-CoV-2 RT-LAMP …

Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis

B de Oliveira Coelho, HBS Sanchuki, DL Zanette… - Molecular …, 2021 - Springer
Abstract Background SARS-CoV-2 Reverse Transcription Loop-mediated Isothermal
Amplification (RT-LAMP) colorimetric detection is a sensitive and specific point-of-care …

[HTML][HTML] Rapid and visual detection of 2019 novel coronavirus (SARS-CoV-2) by a reverse transcription loop-mediated isothermal amplification assay

C Yan, J Cui, L Huang, B Du, L Chen, G Xue… - Clinical Microbiology …, 2020 - Elsevier
Objective To evaluate a reverse transcription loop-mediated isothermal amplification (RT-
LAMP) assay for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV …

Validation of a single-step, single-tube reverse transcription loop-mediated isothermal amplification assay for rapid detection of SARS-CoV-2 RNA

JYH Lee, N Best, J McAuley… - Journal of medical …, 2020 - microbiologyresearch.org
Introduction. The SARS-CoV-2 pandemic of 2020 has resulted in unparalleled requirements
for RNA extraction kits and enzymes required for virus detection, leading to global …