Regulation of the Transposase of Tn4652by the Transposon-Encoded Protein TnpC

R Hõrak, M Kivisaar - Journal of bacteriology, 1999 - Am Soc Microbiol
Transposition is a DNA reorganization reaction potentially deleterious for the host. The
frequency of transposition is limited by the amount of transposase. Therefore, strict …

Transcription from Fusion Promoters Generated during Transposition of Transposon Tn4652 Is Positively Affected by Integration Host Factor in Pseudomonas putida

R Teras, R Hõrak, M Kivisaar - Journal of bacteriology, 2000 - Am Soc Microbiol
We have previously shown that both ends of the Tn 3 family transposon Tn 4652 contain
integration host factor (IHF) binding sites and that IHF positively regulates expression of the …

Target Site Selection of Pseudomonas putida Transposon Tn4652

PA Kivistik, M Kivisaar, R Horak - Journal of bacteriology, 2007 - Am Soc Microbiol
We analyzed the target preferences of a Tn 3 family transposon Tn 4652. Alignment of 93
different insertion sites revealed a consensus sequence which resembles that of Tn 3 …

Transposition of Tn1000: in vivo properties

MM Tsai, RY Wong, AT Hoang… - Journal of …, 1987 - Am Soc Microbiol
Transposition mediated by the Tn1000 transposase was investigated by using transposon
variants carrying synthetic or wild-type termini but no intact Tn1000 genes. Transposon …

Characterization of the Tn5 transposase and inhibitor proteins: a model for the inhibition of transposition

NB de la Cruz, MD Weinreich, TW Wiegand… - Journal of …, 1993 - Am Soc Microbiol
Tn5 is a composite transposon consisting of two IS50 sequences in inverted orientation with
respect to a unique, central region encoding several antibiotic resistances. The IS50R …

Specific nicking at the 3′ ends of the terminal inverted repeat sequences in transposon Tn3 by transposase and an E. coli protein ACP

T Maekawa, K Yanagihara, E Ohtsubo - Genes to Cells, 1996 - Wiley Online Library
Background: Tn3, a bacterial transposon, carries tnpA gene encoding transposase which is
essential for its transposition. The transposition of Tn3 has been reproduced in vitro in a cell …

IHF is the limiting host factor in transposition of Pseudomonas putida transposon Tn4652 in stationary phase

H Ilves, R Hõrak, R Teras, M Kivisaar - Molecular microbiology, 2004 - Wiley Online Library
Transpositional activity of mobile elements is not constant. Conditional regulation of host
factors involved in transposition may severely change the activity of mobile elements. We …

Tn5 transposase loops DNA in the absence of Tn5 transposon end sequences

CD Adams, B Schnurr, D Skoko, JF Marko… - Molecular …, 2006 - Wiley Online Library
Transposases mediate transposition first by binding specific DNA end sequences that define
a transposable element and then by organizing protein and DNA into a highly structured and …

Identification of transposition proteins encoded by the bacterial transposon Tn7

KA Orle, NL Craig - Gene, 1990 - Elsevier
The bacterial transposon, Tn7, encodes an elaborate array of transposition genes,
tnsABCDE. We report here the direct identification of the TnsA, TnsB, TnsC and TnsD …

Identification of transposition proteins encoded by the bacterial transposon Tn7

KA Orle, NL Craig - Gene, 1991 - Elsevier
The bacterial transposon, Tn7, encodes an elaborate array of transposition genes,
tnsABCDE. We report here the direct identification of the TnsA, TnsB, TnsC and TnsD …